Albumin Antibody (5B11) - BSA Free

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 5B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Albumin Antibody (5B11) - BSA Free Summary

• Description: Novus Biologicals Mouse Albumin Antibody (5B11) - BSA Free (NBP2-23495) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Native HSA isolated from human plasma adsorbed onto aluminum hydroxide gel
• Epitope: Clone 01 differs from 192-02 and Clone 05, but overlap as determined by inhibition ELISA.
• Specificity: Clone 5B11 is specific for native and denatured human serum albumin (HSA)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALB
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Clone 5B11 reacts strongly with native and denatured HSA. A strong reaction is seen in ELISA with native or denatured HSA directly coated onto the microtiter well, and also in sandwich ELISA in combination with a polyclonal antibody against HSA . In Western blotting after SDS-PAGE, Clone 5B11 reacts with native and denatured HSA in non-reduced form only.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Albumin Antibody (5B11) - BSA Free

• ALB
• Albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Human serum albumin (HSA) consists of a single polypeptide chain of 584 amino acids, stabilized by 17 disulfide bridges. HSA is the most common protein in serum, it is produced in the liver and the concentration in serum is 35-50 mg/ml. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. The molecular weight is 67.5 kDa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALB