ALAS2 Antibody (4D8) - Azide and BSA Free

Western Blot: ALAS2 Antibody (4D8) [H00000212-M02] - ALAS2 monoclonal antibody (M02), clone 4D8. Western Blot analysis of ALAS2 expression in Raw 264.7 ( Cat # L024V1 ).

ELISA: ALAS2 Antibody (4D8) [H00000212-M02] - Detection limit for recombinant GST tagged ALAS2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 4D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ALAS2 Antibody (4D8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ALAS2 (NP_000023, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGIGRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFK
• Specificity: ALAS2 - aminolevulinate, delta-, synthase 2 (sideroblastic/hypochromic anemia) (4D8)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALAS2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ALAS2 Antibody (4D8) - Azide and BSA Free

• 5-aminolevulinic acid synthase 2,5-aminolevulinate synthase, erythroid-specific, mitochondrial
• ALASE
• ALAS-E
• aminolevulinate, delta-, synthase 2 (sideroblastic/hypochromic anemia)
• aminolevulinate, delta-, synthase 2
• ANH1
• ASBXLDPP
• Delta-ALA synthase 2
• delta-ALA synthetase
• Delta-aminolevulinate synthase 2
• EC 2.3.1.37
• FLJ93603
• XLSA

Background

The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALAS2
• Entrez:
  • 212(Human)
• Uniprot:
  • NP_000023(Human)