Western Blot: Akt1 Antibody [NB600-608] - Lane 1: NIR MW protein ladder. Lane 2: AKT1, recombinant. Lane 3: AKT1, phosphatase-treated. Lane 4: AKT1, mutant T308A/S473A. Lane 5: AKT2, recombinant. Lane 6: AKT2, ...read more
Immunocytochemistry/ Immunofluorescence: Akt1 Antibody [NB600-608] - Tissue: neonatal rat cardiomyocytes. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT antibody at 1:80 dilution for 1 h at ...read more
Immunohistochemistry: Akt1 Antibody [NB600-608] - Analysis of Tissue: (A) normal colon tissue, (B) colon tumor tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT ...read more
Western Blot: Akt1 Antibody [NB600-608] - Lane 1: NIH/3T3 whole cell lysate. Load: 20 ug per lane. Primary antibody: AKT antibody at 1:500 for overnight at 4C. Secondary antibody: HRP conjugated Gt-a-Rabbit IgG at ...read more
Western Blot: Akt1 Antibody [NB600-608] - Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated Akt1, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated ...read more
AKT1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Cytoplasmic, and nuclear after activation by integrin linked protein kinase 1 (ILK1).
Test in a species/application not listed above to receive a full credit towards a future purchase.
AKT Antibody is suitable for western blotting - detect a single band of the expected apparent molecular weight, immunohistochemistry, Immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecipitation, transfected cell culture, primary cell culture and ELISA.
Expected to cross-react with Mouse, Rat and Chicken based on sequence identity.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Alternate Names for AKT1 Antibody
Protein kinase B
rac protein kinase alpha
RAC-alpha serine/threonine-protein kinase
v-akt murine thymoma viral oncogene homolog 1
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; over expression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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