Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF‑BB (Catalog # 220-BB) ...read more
Akt1 phosphorylated at T308 was detected in immersion fixed CCD‑1070Sk human foreskin fibroblast cell line stimulated with Recombinant Human PDGF‑BB (Catalog # 220-BB) using Mouse Anti-Human Phospho-Akt1 (T308) ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Tris with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Protein A or G purified from hybridoma culture supernatant
Sterile PBS to a final concentration of 0.5 mg/mL.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for AKT1 [p Thr308] Antibody (658320) [Unconjugated]
AKT serine/threonine kinase 1
Protein Kinase B Alpha
Protein kinase B
rac protein kinase alpha
RAC-alpha serine/threonine-protein kinase
Serine-Threonine Protein Kinase
v-akt murine thymoma viral oncogene homolog 1
V-Akt Murine Thymoma Viral Oncogene-Like Protein 1
Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Akt1 is the most widely expressed family member and is frequently activated in a number of carcinomas, including breast, prostate, lung, pancreatic, liver, ovarian, and colorectal cancer. Akt1 is activated in a multistep process that involves the sequential phosphorylation of Thr450 by JNK kinases, Thr308 by PDK1, and Ser473 by PDK2 or mTORC2. Activated Akt1 phosphorylates a wide variety of cytosolic, nuclear, and mitochondrial substrates. Human Akt1 shares 98% aa sequence identity with mouse and rat Akt1. MAB7419 also detects mouse Phospho-Akt1 (T308) in Western Blot.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for AKT1 Antibody (MAB7419). (Showing 1 - 5 of 5 FAQs).
How do I choose secondary antibodies to label the same cells when I have two primary antibodies from the same host?
Use isotype-specific secondary antibodies if the primary antibodies are of different isotypes. You can also make direct conjugates of the primary antibodies by use of antibody labeling kits, dyes, or custom conjugations (please contact Technical Support for custom orders).
Why are many of your antibodies formulated with sodium azide and BSA?
Sodium azide is a preservative which is added to prevent bacterial growth. BSA is added as a protein stabilizer.
Do your HRP-conjugated antibodies contain sodium azide?
No. None of our HRP-conjugated antibodies contain sodium azide as this agent inhibits the activity of HRP.
I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.
At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.
What is the molecular weight of your antibodies?
All IgG antibodies are approximately 150 kDa (each heavy chain is about 50 kDa and each light chain is about 25 kDa).
Altered expression of BCL2 in cancer Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease. For example, too little cell death can promote cell overgrowth a... Read full blog post.
AKT1, Scene 1: The Cell Must Go On Akt1 is a serine/threonine-specific protein kinase involved in many cellular signaling pathways. The major function of this kinase is to mediate cell survival, but it also plays key roles in various other cellular functions such as glycogen synthesis... Read full blog post.
EZH2: Epigenetic Regulation Made Easy! Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i... Read full blog post.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.