AKAP9 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: AKAP9 Antibody [NBP1-89167] - Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & centrosome. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: AKAP9 Antibody [NBP1-89167] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

AKAP9 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EKLELSQRLSDLSEQLKQKHGEISFLNEEVKSLKQEKEQVSLRCRELEIIINHNRAENVQSCDTQVSSLLDGVVTMTSRGAEGSVSKVNKSFGEESKIMVEDKVSFENMTVGEESKQEQLILDHLPSVTKESSLRATQPSENDKLQKE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AKAP9
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  AKAP9 Protein (NBP1-89167PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for AKAP9 Antibody - BSA Free

• A kinase (PRKA) anchor protein (yotiao) 9
• AKAP 350
• AKAP 450
• AKAP350A-kinase anchor protein 450 kDa
• AKAP450A-kinase anchor protein 350 kDa
• AKAP-9
• Centrosome- and Golgi-localized PKN-associated protein
• CG-NAPAKAP 120-like protein
• hgAKAP 350
• HYPERION
• KIAA0803A-kinase anchor protein 9
• MU-RMS-40.16A
• PRKA9AKAP9-BRAF fusion protein
• Protein hyperion
• protein kinase A anchoring protein 9
• Protein kinase A-anchoring protein 9
• Protein yotiao
• YOTIAOkinase N-associated protein

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in many isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AKAP9 Antibody (NBP1-89167)(1)

Publication using NBP1-89167

• So C, Seres K. B, et al. A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Science 2019-06-28 [PMID: 31249032] (ICC/IF, Sheep, Bovine, Mouse, Porcine)

Bioinformatics

• Gene Symbol: AKAP9