AKAP4 Antibody - BSA Free

Western Blot: AKAP4 Antibody [NBP2-81946] - Analysis of AKAP4 in human testis tissue lysate with AKAP4 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry: AKAP4 Antibody - BSA Free [NBP2-81946] - Immunohistochemistry of AKAP4 in human testis tissue with AKAP4 antibody at 5 u/ml.

Immunocytochemistry/ Immunofluorescence: AKAP4 Antibody - BSA Free [NBP2-81946] - Immunofluorescence of AKAP4 in human testis tissue with AKAP4 antibody at 20 u/ml.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AKAP4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AKAP4 Antibody - BSA Free (NBP2-81946) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: AKAP4 antibody was raised against a 17 amino acid peptide near the amino terminus of human AKAP4. The immunogen is located within the first 50 amino acids of AKAP4. Amino Acid Squence: KVDLYNPEGQQDQDRK
• Specificity: AKAP4 antibody is human specific. At least three isoforms are known to exist; this antibody will detect the two longest isoforms.
• Predicted Species: Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AKAP4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse: (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for AKAP4 Antibody - BSA Free

• A kinase (PRKA) anchor protein 4
• AKAP-4
• AKAP82AKAP 82
• A-kinase anchor protein 4
• A-kinase anchor protein 82 kDa
• cancer/testis antigen 99
• CT99
• hAKAP82Fsc1
• HIFSC1
• Major sperm fibrous sheath protein
• p82
• PRKA4
• protein kinase A anchoring protein 4
• Protein kinase A-anchoring protein 4
• testis-specific gene HI

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKAP4