AKAP13 Antibody (5B7) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: AKAP13 Antibody (5B7) [H00011214-M01] - Analysis of monoclonal antibody to AKAP13 on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: AKAP13 Antibody (5B7) [H00011214-M01] - Detection limit for recombinant GST tagged AKAP13 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 5B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

AKAP13 Antibody (5B7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse AKAP13 Antibody (5B7) - Azide and BSA Free (H00011214-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-AKAP13 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: AKAP13 (NP_006729, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKLNPQQAPLYGDCVVTVLLAEEDKAEDDVVFYLVFLGSTLRHCTSTRKVSSDTLETIAPGHDCCETVKVQLCASKEGLPVFVVAEEDFHFVQDEAYDAAQFLATSAGNQ
• Specificity: AKAP13 - A kinase (PRKA) anchor protein 13
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AKAP13
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AKAP13 Antibody (5B7) - Azide and BSA Free

• A kinase (PRKA) anchor protein 13
• AKAP-LbcAKAP-13
• A-kinase anchor protein 13
• A-kinase anchoring protein
• ARHGEF13
• Breast cancer nuclear receptor-binding auxiliary protein
• BRXNon-oncogenic Rho GTPase-specific GTP exchange factor
• c-lbc
• FLJ11952
• FLJ43341
• Guanine nucleotide exchange factor Lbc
• HA-3
• HT31
• Human thyroid-anchoring protein 31
• LBCLBC oncogene
• Lymphoid blast crisis oncogeneProtein kinase A-anchoring protein 13
• p47
• PROTO-LB
• PROTO-LBC

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in at least 3 transcript variants encoding different isoforms containing a dbl oncogene homology (DH) domain and a pleckstrin homology (PH) domain. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions. Therefore, these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway and, in addition, function as protein kinase A-anchoring proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AKAP13 Antibody (H00011214-M01)(1)

Publication using H00011214-M01

• Hu JK, Wang L, Li Y, et al. The mRNA and protein expression of A-kinase anchor proteins 13 in human colorectal cancer. Clin Exp Med. 2009-09-25 [PMID: 19779964]

Bioinformatics

• Gene Symbol: AKAP13
• Entrez:
  • 11214(Human)
• OMIM:
  • 604686(Human)
• Uniprot:
  • NP_006729(Human)