AKAP13 Antibody (5B7)

Images

 
Immunocytochemistry/ Immunofluorescence: AKAP13 Antibody (5B7) [H00011214-M01] - Analysis of monoclonal antibody to AKAP13 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: AKAP13 Antibody (5B7) [H00011214-M01] - Detection limit for recombinant GST tagged AKAP13 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
5B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

AKAP13 Antibody (5B7) Summary

Immunogen
AKAP13 (NP_006729, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag.MKLNPQQAPLYGDCVVTVLLAEEDKAEDDVVFYLVFLGSTLRHCTSTRKVSSDTLETIAPGHDCCETVKVQLCASKEGLPVFVVAEEDFHFVQDEAYDAAQFLATSAGNQ
Specificity
AKAP13 - A kinase (PRKA) anchor protein 13
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AKAP13
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publication using H00011214-M01.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AKAP13 Antibody (5B7)

  • A kinase (PRKA) anchor protein 13
  • AKAP-LbcAKAP-13
  • A-kinase anchor protein 13
  • A-kinase anchoring protein
  • ARHGEF13
  • Breast cancer nuclear receptor-binding auxiliary protein
  • BRXNon-oncogenic Rho GTPase-specific GTP exchange factor
  • c-lbc
  • FLJ11952
  • FLJ43341
  • Guanine nucleotide exchange factor Lbc
  • HA-3
  • HT31
  • Human thyroid-anchoring protein 31
  • LBCLBC oncogene
  • Lymphoid blast crisis oncogeneProtein kinase A-anchoring protein 13
  • p47
  • PROTO-LB
  • PROTO-LBC

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in at least 3 transcript variants encoding different isoforms containing a dbl oncogene homology (DH) domain and a pleckstrin homology (PH) domain. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions. Therefore, these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway and, in addition, function as protein kinase A-anchoring proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKAP13 Antibody (H00011214-M01)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AKAP13
Entrez
OMIM
Uniprot