AK2 Antibody (AT7E7) - BSA Free

Western Blot: AK2 Antibody (AT7E7) [NBP2-50575] - The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: NIH/3T3 cell lysate

Immunocytochemistry/Immunofluorescence: AK2 Antibody (AT7E7) [NBP2-50575] - Analysis of AK2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK2 antibody with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western Blot: AK2 Antibody (AT7E7) [NBP2-50575] - The Tissue lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Kidney tissue lysate Lane 2.: Liver tissue lysate

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: AT7E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AK2 Antibody (AT7E7) - BSA Free Summary

• Description: Novus Biologicals Mouse AK2 Antibody (AT7E7) - BSA Free (NBP2-50575) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human AK2 (1-239aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AK2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for AK2 Antibody (AT7E7) - BSA Free

• adenylate kinase 2
• adenylate kinase 2, mitochondrial
• adenylate kinase isoenzyme 2, mitochondrial
• ADK2
• AK 2
• AK2
• ATP-AMP transphosphorylase 2
• EC 2.7.4
• EC 2.7.4.3

Background

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AK2