AIP/ARA9 Antibody (35-2)

Images

 
Western Blot: AIP/ARA9 Antibody (35-2) [NB100-127] - Detection of Ara9 in mouse lung lysates (30ug) using NB 100-127 at 1:500 with a 1 second exposure.
Flow Cytometry: AIP/ARA9 Antibody (35-2) [NB100-127] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using AIP/ARA9 antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Western Blot: AIP/ARA9 Antibody (35-2) [NB100-127] - Detection on HEK 293T/17 cells. Lane 1: IP ARA9, WB ARA9, Lane 2: WB ARA9 with whole cell lysate. Photo courtesy of Liling Zeng, Boston University.
Simple Western: AIP/ARA9 Antibody (35-2) [NB100-127] - Simple Western lane view shows a specific band for AIP/ARA9 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-P, IP
Clone
35-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

AIP/ARA9 Antibody (35-2) Summary

Immunogen
Bacterially expressed human AIP/ARA9 [UniProt# O00170]
Epitope
Within the FKBP region.
Localization
Cytoplasmic
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AIP
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:200
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:100
Application Notes
This AIP/ARA9 antibody may be used for Flow Cytometry, Chromatin Immunoprecipitation, Immunoprecipitation and Western blot. In WB, a band is seen at 37 kDa, representing AIP/ARA9. It is specific for the FKBP domain. Sequential ChIP reported in research paper with PubMed ID: 21984905. Use in Immunocytochemistry/Immunofluorescence was reported in a scientific publication (PMID: 23702468). For IHC-P, this product has been used at 1:500 - 1:1000 dilution range (PMIDs: 22659247, 25019383, 23940012).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
AIP/ARA9 Lysate (NBL1-07415)
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB100-127 in the following applications:

Publications
Read Publications using
NB100-127 in the following applications:

  • 1 publication
  • 1 publication
  • IHC
    3 publications
  • 7 publications
  • IP
    2 publications
  • WB
    12 publications

Reactivity Notes

Customers have reported success on rat lysate (see review).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.1% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for AIP/ARA9 Antibody (35-2)

  • ARA9
  • FKBP16
  • FKBP37
  • SMTPHN
  • XAP2
  • XAP-2

Background

AIP/ARA9 (AhR-interacting protein /AhR-associated protein 9) is a 37 kD immunophilin-like factor expressed ubiquitously with considerable variation in intracellular levels between different tissues. Its high levels of expression are observed in spleen, thymus, pituitary heart, placenta and skeletal muscle, whereas low expression levels are seen in liver, kidney and lung. AIP/ARA9 plays a positive role in AHR-mediated signaling, possibly by influencing its receptivity for ligand and/or its nuclear targeting. It was originally identified as a negative regulator of hepatitis B virus X-associated protein (hence named XAP2 also) and later was shown as Hsp90-associated protein which specifically interacts with AhR leading to regulation of AhR intracellular localization as well as protein stability via inhibition of AhR ubiquitination. AIP/ARA9 was also shown to interact with other signal transduction proteins like Ga13 and nuclear receptor transcription factors including GR, TR-beta1 and PPAR-alpha. Germline mutations that disrupt AIP/ARA9 have been reported in both familial as well as sporadic pituitary tumors and targeted disruption of this protein in mouse leads to cardiac malformation and embryonic lethality. Defects in AIP/ARA9 are the cause of growth hormone-secreting pituitary adenoma (GHSPA), ACTH-secreting pituitary adenoma (ASPA) and prolactin-secreting pituitary adenoma (PSPA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-P, IP

Publications for AIP/ARA9 Antibody (NB100-127)(24)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 6 applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ChIP
(1)
ICC/IF
(1)
IHC
(3)
IHC-P
(7)
IP
(2)
WB
(12)
All Applications
Filter By Species
Human
(17)
Mouse
(3)
Rat
(5)
Primate
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB100-127 Applications Species
Ritvonen E, Pitkanen E, Karppinen A et al. Impact of AIP and inhibitory G protein alpha 2 proteins on clinical features of sporadic GH-secreting pituitary adenomas. Eur. J. Endocrinol. Feb 1 2017 [PMID: 27998919] (IHC, Human) IHC Human
Rotondi S, Modarelli A, Oliva MA et al. Expression of Peroxisome Proliferator-Activated Receptor alpha (PPARalpha) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate in GH3 cells. Mol. Cell. Endocrinol. 2016 Feb 10 [PMID: 26872613] (IHC-P, Human) IHC-P Human
Iacovazzo D, Carlsen E, Lugli F et al. Factors predicting pasireotide responsiveness in somatotroph pituitary adenomas resistant to first generation somatostatin analogues: an immunohistochemical study. Eur. J. Endocrinol. 2015 Nov 19 [PMID: 26586796] (IHC-P, Human) IHC-P Human
Beckers A, Lodish M, Giampaolo T et al. X-linked acrogigantism (X-LAG) syndrome: clinical profile and therapeutic responses Endocr. Relat. Cancer. 2015 Feb 24 [PMID: 25712922] (IHC-P, Human)

Details:
AIP/ARA9 antibody used for Immunohistochemistry staining on formalin-fixed, paraffin embedded human pituitary tumors at a dilution of 1:2,000 using Benchmark Ventana automated instrument.
IHC-P Human
Denes J, Kasuki L, Trivellin G et al. Regulation of Aryl Hydrocarbon Receptor Interacting Protein (AIP) Protein Expression by MiR-34a in Sporadic Somatotropinomas PLoS ONE. 2015 Feb 09 [PMID: 25658813] (WB, IHC-P, Human, Rat)

Details:
AIP/ARA9 antibody used for WB and IHC-P on human and rat samples. IHC-P performed on GH-secreting pituitary tumors from human patients and normal pituitary sections emplying a primary antibody dilution of 1:500 with DAB detection (Fig 1). WB analysis carried out on the lysates of HEK293 and GH3 cells after 48 hours of transfection with miR-34a and anti-miR-34a (Fig 6).
WB, IHC-P Human, Rat
Mian C, Ceccato F, Barollo S et al. AHR Over-Expression in Papillary Thyroid Carcinoma: Clinical and Molecular Assessments in a Series of Italian Acromegalic Patients with a Long-Term Follow-Up. PLoS ONE 2014 Jul 15 [PMID: 25019383] (IHC-P, Human)

Details:
AIP/ARA9 antibody (clone 35-2; dilution 1:500) used for IHC-P on human Papillary Thyroid Carcinoma / PTC as well as normal thyroid tissues with normal pituitary samples taken as positive controls.
IHC-P Human
Jaffrain-Rea ML, Rotondi S et al. Somatostatin analogues increase AIP expression in somatotropinomas, irrespective of Gsp mutations. Endocr Relat Cancer. 2013 Sep 16 [PMID: 23940012] (IHC, Human) IHC Human
Formosa R, Xuereb-Anastasi A, Vassallo J. AIP regulates cAMP signalling and growth hormone secretion in GH3 cells. Endocr Relat Cancer 2013 May 23 [PMID: 23702468] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Kasai S, Ishigaki T, Takumi R et al. beta-Catenin signaling induces CYP1A1 expression by disrupting adherens junctions in Caco-2 human colon carcinoma cells Biochim Biophys Acta 2012 Nov 19 [PMID: 23174221] (WB, Human) WB Human
Trivellin G, Butz H, Delhove J et al. MicroRNA miR-107 is overexpressed in pituitary adenomas and inhibits the expression of aryl hydrocarbon receptor-interacting protein in vitro Am J Physiol Endocrinol Metab 2012 Sep [PMID: 22811466] (WB, Human, Rat) WB Human, Rat
Show All 24 Publications.

Reviews for AIP/ARA9 Antibody (NB100-127) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Other.
We have 4 reviews tested in 1 application: WB.

Reviews using NB100-127:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(3)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot AIP/ARA9 NB100-127
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reviewed by:
Anonymous
WB Human 01/30/2017
View

Summary

ApplicationWestern Blot
Sample TestedAdult intestine,T cells
SpeciesHuman
LotE-3

Comments

Commentsreducing, denatured, 10% gel. 2% BSA/TBST blocking
Western Blot AIP/ARA9 NB100-127
Enlarge
reviewed by:
Anonymous
WB Human 08/28/2012
View

Summary

ApplicationWestern Blot
Sample TestedBxPC3, GH3 and SH-SY5Y whole cell lysates
SpeciesHuman
CommentsThe antibody is very good in Western Blot since it detects only one specific band at the expected size (37 KDa) and with a good intensity even when a small amount of proteins was loaded into the gel (range used 20-80 ug).

Blocking

Blocking Details40ml TBS-Tween 20 (0.05%) with 2.5g non-fat milk for 1 hour at RT

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary Descriptiongoat anti-mouse IRDye 800 from Li-Cor
Secondary Concentration1:8000

Details

Detection NotesOdyssey infrared-imaging system (Li-Cor)

Comments

CommentsThe antibody is very good in Western Blot since it detects only one specific band at the expected size (37 KDa) and with a good intensity even when a small amount of proteins was loaded into the gel (range used 20-80 ug).
 
reviewed by:
Western Forte
WB Human 06/27/2012
View

Summary

ApplicationWestern Blot
Sample TestedHEK293 whole cell lysate
SpeciesHuman

Blocking

Blocking Details3% Milk in PBS for 1 hour at RT

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary Descriptionanti-mouse HRP
Secondary Manufacturer Cat#Jackson Immunoresearch
Secondary Concentration1:5000

Details

Detection NotesECL, 2 mins
Western Blot AIP/ARA9 NB100-127
Enlarge
reviewed by:
Kelsey Stockbridge
WB Other 05/27/2011
View

Summary

ApplicationWestern Blot
Sample TestedHela and GH3 (rat pituitary tumour cell line)
SpeciesOther
LotE2
CommentsThe endogenous ARA9 band appears as strong as the ones for the transfected cells because I loaded more proteins on the lanes from untransfected cells becuase I expected much less ARA9 presence but it is still very clearly there.

Blocking

Blocking DetailsBlocking Buffer: 40ml TBS-Tween 20 (2%) with 2g non-fat milk, Blocking Time: overnight, Blocking Temp: 4 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:5000, Incubation Dilution Buffer: TBS-T, Incubation Time: 1.5 hours, Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-Mouse-HRP from Promega, Secondary Ab Dilution Ratio: 1:10,000

Details

Detection NotesDetection Method: ECL and X-ray film, Exposure Time: 1min, Pos.Ctrl: HeLa cells transfected with ARA9 cDNA, Specific Band: 37kDa

Comments

CommentsThe endogenous ARA9 band appears as strong as the ones for the transfected cells because I loaded more proteins on the lanes from untransfected cells becuase I expected much less ARA9 presence but it is still very clearly there.

Product General Protocols

View specific protocols for AIP/ARA9 Antibody (NB100-127): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for AIP/ARA9 Antibody (NB100-127). (Showing 1 - 2 of 2 FAQs).

  1. I am inquiring regarding the above-mentioned antibody against AIP protein. I would like to know if the epitope recognized by the antibody is known.
    • Thank you for your inquiry in regards to this AIP protein antibody. The data I am showing is that it detects between 300-330, but the exact immunogen information is deemed proprietary.
  2. I am working with ARA9 Antibody (35-2, catalog code NB100-127) and I would like to know against what particular region of ARA9 is this antibody designed specifically? I would appreciate if you send me the sequence of ARA9 that this antibody recognizes.
    • The immunogen used to produce this antibody was full length protein. The antibody binds specifically to the FKBP domain of the ARA9 protein.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on AIP/ARA9.

Aryl Hydrocarbon Signaling: AIP, AhR, ARNT, BMAL1 and more...
AH receptor-interacting protein (AIP) is a 37 kD immunophilin-like factor found in a variety of tissues with expression levels ranging from high (spleen, thymus, pituitary heart, placenta and skeletal muscle) to low (liver, kidney and lung). It mediat...  Read full blog post.

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Anonymous
01/30/2017
Application: WB
Species: Human

Anonymous
08/28/2012
Application: WB
Species: Human

 
Western Forte
06/27/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol AIP
Entrez
Uniprot