AIM2 Antibody

Western Blot: AIM2 Antibody [H00009447-B01P] - Analysis of AIM2 expression in transfected 293T cell line by AIM2 polyclonal antibody. Lane 1: AIM2 transfected lysate(37.73 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: AIM2 Antibody [H00009447-B01P] - Analysis of purified antibody to AIM2 on HeLa cell. (antibody concentration 10 ug/ml)

Product Discontinued

AIM2 Antibody Summary

• Immunogen: AIM2 (NP_004824, 1 a.a. - 343 a.a.) full-length human protein. MESKYKEILLLTGLDNITDEELDRFKFFLSDEFNIATGKLHTANRIQVATLMIQNAGAVSAVMKTIRIFQKLNYMLLAKRLQEEKEKVDKQYKSVTKPKPLSQAEMSPAASAAIRNDVAKQRAAPKVSPHVKPEQKQMVAQQESIREGFQKRCLPVMVLKAKKPFTFETQEGKQEMFHATVATEKEFFFVKVFNTLLKDKFIPKRIIIIARYYRHSGFLEVNSASRVLDAESDQKVNVPLNIIRKAGETPKINTLQTQPLGTIVNGLFVVQKVTEKKKNILFDLSDNTGKMEVLGVRNEDTMKCKEGDKVRLTFFTLSKNGEKLQLTSGVHSTIKVIKAKKKT
• Specificity: AIM2 - absent in melanoma 2,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: AIM2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate for western blot. This product is also useful for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AIM2 Antibody

• absent in melanoma 2PYHIN4
• AIM2
• Gm1313
• Ifi210
• interferon-inducible protein AIM2
• PYHIN4

Background

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AIM2 Antibody (H00009447-B01P)(4)

Publications using H00009447-B01P

• Ansari MA, Singh VV, Dutta S et al. Constitutive Interferon-Inducible Protein 16-Inflammasome Activation during Epstein-Barr Virus Latency I, II, and III in B and Epithelial Cells. J Virol. 2013-05-29 [PMID: 23720728]
• Wang LJ, Hsu CW, Chen CC et al. Interactome-wide analysis identifies end-binding protein 1 as a crucial component for the speck-like particle formation of activated AIM2 inflammasomes. Mol Cell Proteomics. 2012-08-06 [PMID: 22869553]
• Lee J, Li L, Gretz N et al. Absent in Melanoma 2 (AIM2) is an important mediator of interferon-dependent and -independent HLA-DRA and HLA-DRB gene expression in colorectal cancers. Oncogene. 2011-08-01 [PMID: 21804607]
• Fernandes-Alnemri T, Yu JW, Datta P et al. AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. Nature. 2009-03-26 [PMID: 19158676]

Bioinformatics

• Gene Symbol: AIM2
• Entrez:
  • 9447(Human)
• Uniprot:
  • NP_004824(Human)