AIF Antibody

Product Discontinued

AIF Antibody Summary

• Immunogen: Peptide corresponding to amino acids 517 - 531 of human AIF.
• Localization: Mitochondrial intermembrane space. Translocated to the nucleus upon induction of apoptosis.
• Specificity: NB100-66560 recognises apoptosis inducing factor (AIF). The reacts with an internal region of AIF. AIF is widely expressed in human tissues and localises in the mitochondria. AIF translocates to the nucleus upon induction of apoptosis.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AIFM1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.25 ug/ml-1 ug/ml
• Application Notes: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Control:

  AIF-1/Iba1 Overexpression Lysate

Reactivity Notes

Reacts with Human. Cross reacts with: Rat and Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: IgG purified

Alternate Names for AIF Antibody

• AIF
• AIFapoptosis-inducing factor 1, mitochondrial
• AIFM1
• apoptosis-inducing factor, mitochondrion-associated, 1
• COXPD6
• MGC111425
• PDCD8
• PDCD8striatal apoptosis-inducing factor
• programmed cell death 8 (apoptosis-inducing factor)
• Programmed cell death protein 8

Background

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase 9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AIFM1
• Entrez:
  • 9131(Human)
• Uniprot:
  • O95831(Human)