AICDA Antibody

Images

 
Western Blot: AICDA Antibody [NBP1-77235] - Western blot analysis of AID in Ramos whole cell lysate with anti-AID using IMG-5410 at 2 ug/ml in either the (A) absence or (B) presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: AICDA Antibody [NBP1-77235] - Immunocytochemistry analysis of AID in Ramos cells using NBP1-77235 at 10 ug/ml.
Immunofluorescence: AICDA Antibody [NBP1-77235] - Ramos cells with AICDA antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

AICDA Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human AID. The immunogen is located within the last 50 amino acids of AID.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AICDA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunofluorescence
Application Notes
This antibody can be used for detection of AID by Western blot at 1 - 2 mg/ml. Whole cell lysate from Ramos cells can be used as positive control. Anti-AID is human and mouse reactive.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
AICDA Peptide (NBP1-77235PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AICDA Antibody

  • activation-induced cytidine deaminase
  • AICDA
  • AID
  • AIDCytidine aminohydrolase
  • Arp2
  • CDA2
  • HIGM2
  • HIGM2EC 3.5.4.5
  • integrated into Burkitt's lymphoma cell line Ramos

Background

Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AICDA
Entrez
Uniprot