AICDA Antibody - BSA Free

Western Blot: AICDA Antibody [NBP1-77235] - Western blot analysis of AID in Ramos whole cell lysate with anti-AID using IMG-5410 at 2 ug/ml in either the (A) absence or (B) presence of blocking peptide.

Immunocytochemistry/Immunofluorescence: AICDA Antibody [NBP1-77235] - Ramos cells with AICDA antibody at 20 ug/mL.

Immunocytochemistry/Immunofluorescence: AICDA Antibody [NBP1-77235] - Immunocytochemistry analysis of AID in Ramos cells using NBP1-77235 at 10 ug/ml.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AICDA Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human AID. The immunogen is located within the last 50 amino acids of AID. Amino Acid Squence: EVDDLRDAFRTLGL
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AICDA
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Western Blot 2 ug/ml
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  AICDA Peptide (NBP1-77235PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for AICDA Antibody - BSA Free

• activation-induced cytidine deaminase
• AICDA
• AID
• AIDCytidine aminohydrolase
• Arp2
• CDA2
• HIGM2
• HIGM2EC 3.5.4.5
• integrated into Burkitt's lymphoma cell line Ramos

Background

Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AICDA
• Uniprot:
  • AAM95402(Human)