AHCYL1 Antibody - BSA Free

Western Blot: AHCYL1 Antibody [NBP2-98585] - Anti-AHCYL1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 59 kDa

Immunohistochemistry-Paraffin: AHCYL1 Antibody [NBP2-98585] - Immunochemical staining of human AHCYL1 in human pancreas with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: AHCYL1 Antibody [NBP2-98585] - Immunochemical staining of human AHCYL1 in human duodenum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: AHCYL1 Antibody [NBP2-98585] - AHCYL1 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 4 ul anti-AHCYL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-AHCYL1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 59 kDa

Immunocytochemistry/Immunofluorescence: AHCYL1 Antibody [NBP2-98585] - Immunofluorescence staining of AHCYL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98585 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

AHCYL1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human AHCYL1 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AHCYL1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for AHCYL1 Antibody - BSA Free

• adenosylhomocysteinase-like 1
• AdoHcyase 2
• DCAL
• DC-expressed AHCY-like molecule
• dendritic cell expressed AHCY-like protein
• inositol 14,5-trisphosphate receptor-binding protein
• IRBIT
• PRO0233
• putative adenosylhomocysteinase 2
• S-adenosyl homocysteine hydrolase homolog
• S-adenosylhomocysteine hydrolase-like 1
• S-adenosylhomocysteine hydrolase-like protein 1
• S-adenosyl-L-homocysteine hydrolase 2
• XPVKONAEC 3.3.1.1

Background

adenosylhomocysteinase-like 1;PRO0233;XPVKONA;S-adenosyl homocysteine hydrolase homolog;IRBIT;AdoHcyase 2;S-adenosylhomocysteine hydrolase-like 1;DC-expressed AHCY-like molecule;DCAL;EC 3.3.1.1;dendritic cell expressed AHCY-like protein;S-adenosylhomocysteine hydrolase-like protein 1;inositol 1,4,5-trisphosphate receptor-binding protein;S-adenosyl-L-homocysteine hydrolase 2;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AHCYL1