Aggrecan Antibody (MK-302)

Product Discontinued

Aggrecan Antibody (MK-302) Summary

• Description: Relevant Entrez Gene Numbers: NM_001135.2;NM_013227.2
• Immunogen: Chondroitinase ABC-digested adult human aggrecan.
• Marker: Chondrocyte Marker
• Specificity: Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Native proteoglycans do not react with MAB2035. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. No cross-reactivity with dermatan sulfate, keratan sulfate or chondroitin-4-sulfate.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACAN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:5000
  • Radioimmunoassay 1:100-1:200
  • Western Blot 1:100-1:2000
• Application Notes: Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. {0.5U/mL in 100mM Tris-HCL, 30 minutes room temperature} A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: No Preservative
• Purity: Unpurified

Notes

Relevant Entrez Gene Numbers: NM_001135.2;NM_013227.2

Alternate Names for Aggrecan Antibody (MK-302)

• ACAN
• AGC1
• AGC1SEDK
• aggrecan core protein
• Aggrecan
• Cartilage-specific proteoglycan core protein
• Chondroitin sulfate proteoglycan 1
• Chondroitin sulfate proteoglycan core protein 1
• CSPCP
• CSPG1
• CSPG1aggrecan 1
• CSPGCP
• large aggregating proteoglycan
• MSK16
• MSK16AGCAN
• SEDK

Background

This aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACAN
• Uniprot:
  • P16112()