Immunocytochemistry/ Immunofluorescence: Aggrecan Antibody (CS-56) [NB120-11570] - Coronal section of the developing cerebral cortex of an embryonic day 16 rat stained with Monoclonal Anti-Chondroitin Sulfate followed ...read more
Immunohistochemistry: Aggrecan Antibody (CS-56) [NB120-11570] - Long-term load duration decreased expression of NP matrix macromolecules in porcine disc NP cells. Immunohistochemistry staining of aggrecan and collagen ...read more
Immunohistochemistry-Frozen: Aggrecan Antibody (CS-56) [NB120-11570] - staining of a frozen section of normal rabbit aorta. Data supplied by Z. Gallis, McGill University, Montreal.
Electron Microscopy: Aggrecan Antibody (CS-56) [NB120-11570] - Normal rabbit aorta, Lowicryl K4M thin section, stained with Monoclonal Anti-Chondroitin Sulfate (NB120-11570) and Goat Anti-Mouse IgM (u-chain specific) 10 ...read more
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Aggrecan Antibody (CS-56) Summary
Immunogen
Ventral membranes of chicken gizzard fibroblasts.
Marker
Chondrocyte Marker
Specificity
The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
ACAN
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Rabbit reactivity reported in scientific literature (PMID:33032177).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.09% Sodium Azide
Purity
Unpurified
Alternate Names for Aggrecan Antibody (CS-56)
ACAN
AGC1
AGC1SEDK
aggrecan core protein
Aggrecan
Cartilage-specific proteoglycan core protein
Chondroitin sulfate proteoglycan 1
Chondroitin sulfate proteoglycan core protein 1
CSPCP
CSPG1
CSPG1aggrecan 1
CSPGCP
large aggregating proteoglycan
MSK16
MSK16AGCAN
SEDK
Background
Many cellular activities depend on the interaction of cells with the surrounding extracellular matrix (ECM). Most cells, in intact tissue and in culture, are attached to an ECM. Epithelial cells are associated with the basement membrane; fibroblastic cells are usually embedded in a pericellular mesh of fibrils, and tissue culture cells usually grow on a substrate which is covered by various ECM components. Studies have indicated that the matrix or its various isolated components provide not only adhesive surfaces for cells to grow on but also have effects on the rate of cell growth, mobility, morphogenesis and differentiation. Within the ECM several glycoproteins and proteoglycans have been identified. It has been proposed that the different constituents interact with each other in a rather complex fashion. The poor antigenicity of proteoglycans especially their glycoaminoglycan (GAG) moieties make it difficult to localize these molecules in tissue and cell culture. Monoclonal Anti-Chondroitin Sulfate can be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Aggrecan Antibody (NB120-11570). (Showing 1 - 1 of 1 FAQs).
Can I use NB120-11570 for IHC-paraffin?
Our product NB120-11570 has not yet been tested in paraffin embedded tissues, only in frozen tissues. So we unfortunately have not been able to add IHC-P to our verified list of applications for this product.
Aches & Pains: Aggrecan in Joint Disease and Osteoarthritis Aggrecan is essential for the normal function of articular cartilage and intervertebral discs. Aggrecan provides the ability for the tissues to withstand compressive loading. This property is dependent on both the high charge density endowed by its nu... Read full blog post.
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