AG-2 Antibody (10E2)

Images

 
Western Blot: AG-2 Antibody (10E2) [NBP2-27175] - Analysis of human stomach in the A) absense and B) presence of immunizing peptide, C) mouse stomach, D) rat stomach tissue lysate, and E) HCT-116 cell lysate using AGR2 ...read more
Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - IHC-P detection of AGR2 protein in a human ovarian cancer section using 5 ug/ml concentration of AGR2 antibody. The representative image shows AGR2 ...read more
Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - Formalin-fixed, paraffin-embedded human breast stained with AGR2 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue ...read more
Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - Paraffin-embedded human small intestine tissue stained with AGR2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue ...read more
Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - Formalin-fixed, paraffin-embedded human spleen tissue stained with 5 ug/ml concentration of AGR2 antibody.
Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - IHC-P detection of AGR2 protein in a section of human bladded cancer using 5 ug/ml concentration of AGR2 antibody. The AGR2 immunoreactivity was ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Ca, Eq, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clone
10E2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

AG-2 Antibody (10E2) Summary

Immunogen
A portion of amino acids 50-100 of human AGR2 was used as the immunogen for the antibody.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AGR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 4-6ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Positive Control
AG-2 Lysate (NBP2-07625)

Reactivity Notes

Orangutan, Panda and Platypus. Immunogen sequence similarity with other species: Xenopus (82%), Chicken (83%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for AG-2 Antibody (10E2)

  • AG2
  • AG-2
  • AG2member 17
  • AGR2
  • anterior gradient 2 homolog (Xenopus laevis)
  • anterior gradient homolog 2 (Xenopus laevis)
  • GOB-4
  • hAG-2
  • HPC8
  • PDIA17
  • secreted cement gland homolog

Background

hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR1 and AGR2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development. Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-27175AF405
Alexa Fluor 488 Labeled NBP2-27175AF488
Alexa Fluor 647 Labeled NBP2-27175AF647
Alexa Fluor 700 Labeled NBP2-27175AF700
Biotin Labeled NBP2-27175B
DyLight 350 Labeled NBP2-27175UV
DyLight 405 Labeled NBP2-27175V
DyLight 488 Labeled NBP2-27175G
DyLight 550 Labeled NBP2-27175R
DyLight 650 Labeled NBP2-27175C
DyLight 680 Labeled NBP2-27175FR
DyLight 755 Labeled NBP2-27175IR
HRP Labeled NBP2-27175H

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Bioinformatics

Gene Symbol AGR2
Entrez
Uniprot