Recombinant Human AG-2/AGR2 His Protein

SDS-Page: Recombinant Human AG-2/AGR2 Protein [NBC1-18502] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human AG-2/AGR2 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 21-175 of Human AG-2
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSRDTT VKPGAKKDTK DSRPKLPQTL SRGWGDQLIW TQTYEEALYK SKTSNKPLMI IHHLDECPHS QALKKVFAEN KEIQKLAEQF VLLNLVYETT DKHLSPDGQY VPRIMFVDPS LTVRADITGR YSNRLYAYEP ADTALLLDNM KKALKLLKTE L

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: AGR2
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 21.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM EDTA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human AG-2/AGR2 His Protein

• AG2
• AG-2
• AG2member 17
• AGR2
• anterior gradient 2 homolog (Xenopus laevis)
• anterior gradient homolog 2 (Xenopus laevis)
• GOB-4
• hAG-2
• HPC8
• PDIA17
• secreted cement gland homolog

Background

AGR2(Anterior gradient 2 homolog) is the human orthologue of the secreted Xenopus laevis Anterior Gradient protein(XAG-2). This is a small, possibly secreted molecule of yet weakly defined functions that is widely expressed in human tissues. Expression of AGR2 shows a positive correlation with expression of estrogen receptor in breast carcinoma and a negative correlation with expression of EGF receptor. Recombinant human AGR2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for AG-2/AGR2 Recombinant Protein (NBC1-18502). (Showing 1 - 1 of 1 FAQ).

1. I would like some details about AGR2 protein. How did you produce this protein? Did you test if it functional on cells?
   • Recombinant human AGR2 protein (NBC1-18502) fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. As far as the applications are concerned, this product can only be used for SDS PAGE and ELISA and to our knowledge it is not suitable for functional assays.

Bioinformatics

• Gene Symbol: AGR2