Mouse AG-2/AGR2 ELISA Kit (Colorimetric) Summary
| Specificity |
This assay has high sensitivity and excellent specificity for detection of Mouse AG-2. No significant cross-reactivity or interference between Mouse AG-2 and analogues was observed. |
| Standard Curve Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 10% |
| Intra-Assay |
CV% < 8% |
| Sample Volume |
50-100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
AGR2 |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
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- Adhesive Strip (For 96 wells)
- Assay Plate (12 x 8 coated Microwells)
- Biotin-antibody (100 x concentrate)
- Biotin-antibody Diluent
- HRP-avidin (100 x concentrate)
- HRP-avidin Diluent
- Instruction Manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
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Alternate Names for Mouse AG-2/AGR2 ELISA Kit (Colorimetric)
Background
Anterior gradient homolog 2 (AGR2) is a proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. AGR2 is expressed in a number of different tumor types and high levels of AGR2 correlate with downregulation of the p53 response. AGR2 is being investigated for its use as a potential diagnostic and therapeutic target in various cancers, and AGR2 antibodies are important tools for cancer research.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for AG-2/AGR2 ELISA Kit (NBP2-77027). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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