Afadin/AF-6 Antibody [HRP]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: HRP

Afadin/AF-6 Antibody [HRP] Summary

• Immunogen: E. coli-derived recombinant human Afadin/AF-6
  Asn217-Pro392
  Accession # P55196
• Specificity: Detects human Afadin/AF-6 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human METTL-11A is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: MLLT4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Afadin/AF-6 Antibody [HRP]

• AF6
• AF-6
• AF64
• AFAD
• Afadin
• FLJ34371
• MLLT4
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 4

Background

AF6/Afadin was identified as part of a fusion with MLL (mixed lineage leukemia) in a t(6;11) genetic translocation. AF6/Afadin is also known as afadin (a splicing variant of AF6/Afadin found at adherins junction). AF6/afadin is an actin filament binding protein that binds to nectin at cadherin-based cell-cell adherens junctions and regulates cell-cell adhesion by mediating Ras signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MLLT4