Afadin/AF-6 Antibody (851204) [Unconjugated]

Images

 
Western blot shows lysates of IMR‑32 human neuroblastoma cell line and SK‑OV‑3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Afadin/AF-6 Monoclonal Antibody ...read more
Afadin/AF-6 was detected in immersion fixed BEAS2B human bronchial epithelial cell line using Mouse Anti-Human Afadin/AF-6 Monoclonal Antibody (Catalog # MAB78291) at 25 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
851204
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

Afadin/AF-6 Antibody (851204) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Afadin/AF-6
Asn217-Pro392
Accession # P55196
Specificity
Detects human Afadin/AF-6 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MLLT4
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Afadin/AF-6 Antibody (851204) [Unconjugated]

  • AF6
  • AF-6
  • AF64
  • AFAD
  • Afadin
  • FLJ34371
  • MLLT4
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 4

Background

Afadin, also known as MLLT-4 (Myeloid/Lymphoid or mixed-lineage Leukemia Translocated to Ch 4) and AF-6,  is a 200-210 kDa nectin and F-actin-binding intracellular protein. It is ubiquitously expressed, and serves as an intermediary that links nectin with the actin cytoskeleton in tight and adherens junctions (AJs). Afadin is involved in the nectin-mediated recruitment of catenins to zonule adherens complexes, and in the recruitment of ZO-1 to the apical side of AJs for the purpose of forming tight junctions. Human Afadin is 1824 amino acids (aa) in length. The protein contains two Ras-association (RA) domains (aa 20-348), a phosphoprotein FHA domain (aa 426‑492), a Vav2 Ras-activating "dilute" domain (aa 668-908), one PDZ domain that binds protein C-termini (aa 1007-1093), and three proline-rich regions (aa 1346‑1708). There are at least fourteen utilized Ser/Thr phosphorylation sites and two Tyr phosphorylation sites. There are at least seven alternative splice variants are reported for Afadin. There is a 190 kDa isoform that shows a 12 aa insert after Arg1605 coupled to a four aa substitution for aa 1650-1824. Other isoforms contain scattered deletions of one to three aa coupled to major aa changes. Five isoforms share a deletion of aa 393-407, with four of these containing a 24 aa substitution for aa 1605-1824, a 73 aa substitution for aa 1747-1824, an 11 aa substitution for aa 680-1824, and a deletion of aa 1683-1746, respectively. A final isoform possesses a four aa substitution for aa 1650-1824. Over aa 217-392, human Afadin shares 94% aa sequence identity with mouse Afadin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MLLT4
Uniprot