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ADRM1 Antibody

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Western Blot: ADRM1 Antibody [NBP1-30447] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADRM1 antibody NBP1-30447 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Biological Strategies: Western Blot: ADRM1 Antibody [NBP1-30447] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ADRM1 antibody ...read more
Immunohistochemistry-Paraffin: ADRM1 Antibody [NBP1-30447] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ADRM1 used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB
Independent Antibodies: Immunoprecipitation: ADRM1 Antibody [NBP1-30447] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

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ADRM1 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 357 and 407 of human adhesion regulating molecule 1 using the numbering given in entry NP_008933.2 (GeneID 11047).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADRM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:500 -1:2000
  • Immunohistochemistry-Paraffin 1:500 -1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Control
ADRM1 Knockout 293T Cell Lysate
Publications
Read Publications using
NBP1-30447 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ADRM1 Antibody

  • 000 surface antigen
  • 110 kDa cell membrane glycoprotein
  • adhesion regulating molecule 1
  • Adhesion-regulating molecule 1
  • ARM1
  • ARM-1
  • Gp110
  • GP110hRpn13
  • M(r) 110
  • MGC29536
  • proteasomal ubiquitin receptor ADRM1
  • Proteasome regulatory particle non-ATPase 13
  • regulatory particle non-ATPase 13
  • Rpn13 homolog
  • Rpn13

Background

The protein encoded by this gene is an integral plasma membrane protein which promotes cell adhesion. The encoded protein is thought to undergo O-linked glycosylation. Expression of this gene has been shown to be induced by gamma interferon in some cancer cells. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: S5a/Angiocidin Antibody [NBP2-37888] - Analysis in control (vector only transfected HEK293T lysate) and PSMD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human liver.
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Western Blot: Rpn2 Antibody [NBP2-38323] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: Rpn2 Antibody [NBP2-38323] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & actin filaments.
Immunohistochemistry-Paraffin: Rpn2 Antibody [NBP2-38323] - Staining of human liver.
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Western Blot: Ribophorin II Antibody [NBP1-80835] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: Ribophorin II Antibody [NBP1-80835] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
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Western Blot: TPPP/p25 Antibody [NBP2-34031] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral cortex tissue.
Immunocytochemistry/Immunofluorescence: TPPP/p25 Antibody [NBP2-34031] - Staining of human cell line A549 shows localization to cytosol & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line,PC‑12 rat adrenal pheochromocytoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ERK2 at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for ERK2 at approximately 43 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.
Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 19S 10B.
Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human pancreas tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.
Species: Hu, Mu, Rt, Ca, Pm, Pm
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Western Blot: PSMD13 Antibody [NBP1-80961] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: PSMD13 Antibody [NBP1-80961] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & nuclear speckles.
Immunohistochemistry-Paraffin: PSMD13 Antibody [NBP1-80961] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
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Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribophorin I (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ribophorin I.
Immunocytochemistry/Immunofluorescence: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - expression of RPN1 on pca cell lines vs normal. Image from verified customer review.
Immunohistochemistry: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribophorin I mouse monoclonal antibody.
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Immunohistochemistry: CD4 Antibody [NBP1-19371] - Increased CD3+ and CD4+ T-cell occurrence in the brainstem of SHR-72 transgenic rat model for tauopathies. (A-D) Immunofluorescence staining showed CD4+ T-cells in SHR-72 transgenic animals. (E- H) Immunofluorescence staining showed more perivascular than brain parenchyma infiltrating CD4+ T-cells in SHR-72 transgenic animals. PLoS One. 2019 May 23;14(5):e0217216. doi: 10.1371/journal.pone.0217216.
Immunohistochemistry: CD4 Antibody [NBP1-19371] - DBZ inhibits the accumulation of CD4+ T cells and Th2 differentiation in the AAAs. (B) The representation of immunohistochemical staining for CD4+ in abdominal aorta from four groups (left). Bar graphs show the percentage of CD4+ positive cell areas (right; n=3 per group). Bar: 50 um.
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
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Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours (right lane) and 10 uL of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.
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Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.
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Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Simple Western lane view shows lysates of LoVo human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 168 kDa (as indicated) using 2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western Blot: FAT10 Antibody (JJ084-09) [NBP2-67917] - Analysis of Ubiquitin D on Hela cells lysates using anti-Ubiquitin D antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: FAT10 Antibody (JJ084-09) [NBP2-67917] - Staining Ubiquitin D in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: FAT10 Antibody (JJ084-09) [NBP2-67917] - Analysis of paraffin-embedded mouse kidney tissue using anti-Ubiquitin D antibody. Counter stained with hematoxylin.
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INO80 Antibody
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Immunoprecipitation: INO80 Antibody [NBP1-78758] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. AAffinity purified rabbit anti-INO80 antibody used for IP at 6 mcg/mg lysate. INO80 was also immunoprecipitated by rabbit anti-INO80 antibody NBP1-78759 which recognizes a downstream epitope.
Immunoprecipitation: INO80 Antibody [NBP1-78758] - Detection of human INO80 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INO80 antibody NBP1-78758 lot 2 used for IP at 6 ug per reaction. INO80 was also immunoprecipitated by rabbit anti-INO80 antibody NBP1-78759. For blotting immunoprecipitated INO80, NBP1-78759 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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LAMTOR1 Antibody
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Western Blot: c11orf59/LAMTOR1 Antibody [NBP1-71689] - Analysis of c11orf59 in human liver protein.
Immunocytochemistry/Immunofluorescence: c11orf59/LAMTOR1 Antibody [NBP1-71689] - C11orf59 antibody was tested at 1:50 in HepG2 cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).
Immunohistochemistry: c11orf59/LAMTOR1 Antibody [NBP1-71689] - Analysis of C11orf59 in mouse pancreas.
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Western Blot: HN1 Antibody [NBP1-32510] - Wild-type (WT) and HN1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: HN1 Antibody [NBP1-32510] - (30 ug whole cell lysate) A: A431 B: H1299 C: HeLa S3 D: Hep G2 12% SDS PAGE; antibody diluted at 1:1000
Western Blot: HN1 Antibody [NBP1-32510] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HN1 antibody diluted at 1:500.
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Western Blot: p18INK4c/CDKN2C Antibody (18P118 (same as DCS-118)) [NB120-3216] - Analysis of HeLa Cells.
Immunohistochemistry-Paraffin: p18INK4c/CDKN2C Antibody (18P118 (same as DCS-118)) [NB120-3216] - Human tonsil stained with p18 (NB120-3216) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.
Immunoprecipitation: p18INK4c/CDKN2C Antibody (18P118 (same as DCS-118)) [NB120-3216] - Analysis of native IP.
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Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of CFL1 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/Immunofluorescence: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of monoclonal antibody to CFL1 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of monoclonal antibody to CFL1 on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 1.5 ug/ml
(4 Publications)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
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Antibody (5F5)
H00029978-M03
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Generation of transgenic mice that overexpress human ubiquilin-1. Immunoblots of equal amounts of total brain lysates from 12 month-old WT mouse, 12 month-old Ubqln-1 48 transgenic mouse, 12 month-old Ubqln-1 62 transgenic mouse and end stage 15 week-old R6/2 transgenic mouse. The top panel was probed with a monoclonal anti-ubiquilin antibody (Invitrogen antibody clone 3D5E2) and the lower panel with a different monoclonal anti-Ubiquilin 2 antibody (Novus antibody clone 5F5). Note two immunoreactive ubiquilin bands are seen at 70 kDa and at 90 kDa, which we presume is a modified form of ubiquilin. Both blots were also probed for actin to ensure equal loading. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0087513) licensed under a CC-BY licence.
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Confirmation of polyQ-associated proteins from PC-12 cells identified by TAPI. Western blot analysis of TAPI-purified polyQ aggregates from PC-12 cells confirms the presence of several disease-associated proteins only in the Htt-Q74 samples. All proteins migrated near their predicted molecular weights. For control, the TAPI procedure was conducted in parallel on the induced Htt-Q23 cell line (FUS, TDP-43, UBQLN2, HNRNPA1) or the un-induced Htt-Q74 cell line (CLINT1, HSPA8, RAD23B, SGTA). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0136362), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of monoclonal antibody to UBQLN2 on A-431 cell. Antibody concentration 10 ug/ml
(1 Review)
(26 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, KD
ADRM1 Antibody
NBP1-30447
ADRM1 Antibody
Species: Hu
Applications: WB, IHC, IHC-P, IP

Publications for ADRM1 Antibody (NBP1-30447)(3)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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Control Lysate(s)

ADRM1 Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol ADRM1