Adenine Nucleotide Translocator 2 Overexpression Lysate

Images

 
Western Blot: Adenine Nucleotide Translocator 2 Overexpression Lysate (Adult Normal) [NBL1-16073] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adenine ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Adenine Nucleotide Translocator 2 Overexpression Lysate Summary

Description

Adenine Nucleotide Translocator 2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC208949

Accession#: NM_001152

Protein Tag: C-MYC/DDK
Gene
SLC25A5

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
32.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Adenine Nucleotide Translocator 2 Overexpression Lysate

  • adenine nucleotide translocator 2 (fibroblast)
  • Adenine nucleotide translocator 2
  • ADP
  • ADP/ATP carrier protein
  • ADP/ATP translocase 2
  • ANT2AAC2, 2F1
  • ATP carrier protein 2
  • ATP carrier protein, fibroblast isoform
  • solute carrier family 25 (mitochondrial carrier; adenine nucleotidetranslocator), member 5
  • Solute carrier family 25 member 5
  • T2
  • T3ANT 2

Background

ADP/ATP translocase, the most abundant mitochondrial protein, is an integral component of the inner mitochondrial membrane. It facilitates exchange of ADP and ATP between the cytosol and the mitochondria, thereby linking the subcellular compartment of ATP production to those of ATP utilization. SLC25A5 is 1 of at least 3 transcriptionally active ADP/ATP translocase genes in humans (Chen et al., 1990 [PubMed 2157297]). Battini et al. (1987) [PubMed 3031073] cloned an ADP/ATP translocase gene from an Okayama-Berg library derived from SV40-transformed human fibroblasts. Ku et al. (1990) [PubMed 2168878] cloned and sequenced the ANT2 gene. Chen et al. (1990) [PubMed 2157297] isolated 7 ADP/ATP translocase pseudogenes from recombinant human genomic libraries. Each pseudogene sequence had more than 85% identity with the sequence of the translocase cDNA derived from fibroblast mRNA, but each had mutations that precluded synthesis of a functional protein.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SLC25A5