ADAMTS4 Assay Kit (Colorimetric)

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ADAMTS4 Assay Kit (Colorimetric) [KA1497] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Product Details

Summary
Applications Func
Suitable Sample Type
Biological Samples

Order Details

ADAMTS4 Assay Kit (Colorimetric) Summary

Description
The Aggrecanase Activity Assay measures activity of aggrecanases, and is used to screen and characterize aggrecanase inhibitors.
Kit Type
Assay Kit (Colorimetric)
Gene
ADAMTS4

Applications/Dilutions

Publications
Read Publications using KA1497.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Aggrecan-IGD: Tris-buffered solution of 1 µM aggrecan-IGD with additives and preservative.Ready to use
  2. ADAMTS4-Standard: Solution of 0.3 µM recombinant truncated human aggrecanase 1(ADAMTS4 amino acids F213 - A579 with C-terminal His-tag) with additives. Ready to use.
  3. Inhibitor Solution: Inhibitor concentrated with 4 mM Pefabloc, 0.01 mM pepstatin, 0.01 mMleupeptin in 10 mM MES buffer pH 6.0. Ready to use.
  4. Reaction Buffer: Solution of 0.05 M Tris-HCl buffer pH 7.5, 0.15 M NaCl, 5 mM CaCl2, 10 µMZnCl2, 0.05 % Brij 35 with preservative. Ready to use
  5. EDTA Dilution Buffer: Solution of 0.01 M EDTA, 10 mg/ml bovine serum albumin, 0.15 M NaCl,0.02 M Na-phosphate pH 7.2 with preservative. Ready to use
  6. Microtiter Plate: The plate contains 6 x 16 well strips coated with anti-neoepitope ARGSVILantibody. Ready to use.
  7. ELISA Buffer: Solution of 10 mg/ml bovine serum albumin, 0.15 M NaCl, 0.02 M Na-phosphatepH 7.2 with preservative. Ready to use.
  8. ARGSVIL-Peptide Standard: ARGSVIL-peptide at a concentration of 40 nM in EDTA-dilutionbuffer. Ready to use
  9. Antibody-Peroxidase Conjugate: Anti-aggrecan antibody coupled to horseradish peroxidase instabilization solution. The conjugate has to be diluted 100-fold prior to use
  10. Wash Buffer: Bottle contains 25 ml phosphate buffer concentrate which when diluted gives0.02 M Na-phosphate buffer pH 7.2, 0.15 M NaCl, 0.05 % Tween 20.
  11. Detection Solution TMB: Bottle contains 3, 3', 5, 5'-tetramethylbenzidine (TMB)/hydrogenperoxide. Ready to use
  12. Sulfuric Acid: Bottle contains 0.25 M sulfuric acid. Ready to use

Notes

Quality control test: Standard Curve

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ADAMTS4 Assay Kit (Colorimetric)

  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 4
  • ADAM metallopeptidase with thrombospondin type 1 motif, 4
  • ADAM-TS 4
  • ADAMTS2
  • ADAMTS-2
  • ADAMTS4
  • ADAM-TS4
  • ADAMTS-4
  • ADMP-1
  • ADMP-1EC 3.4.24.82
  • Aggrecanase 1
  • aggrecanase-1
  • EC 3.4.24
  • KIAA0688A disintegrin and metalloproteinase with thrombospondin motifs 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Publications for ADAMTS4 Kit (KA1497)(2)

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Bioinformatics

Gene Symbol ADAMTS4