ADAMTS4 Activity Assay Kit (Colorimetric)


ADAMTS4 Assay Kit (Colorimetric) [KA1497] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Product Details

Reactivity Hu, RtSpecies Glossary
Applications Func
Suitable Sample Type
This kit has been tested with synovial fluid samples from calf, rabbit and human. We are recommending the dilutions of the samples to a final concentration of 10-20% in the reaction mix (avoiding the inhibition of the the aggrecanase reaction by undiluted synovial fluid). But, the samples must be serum-, EDTA-, heparin-free. Also, the kits cannot be used for tissues, because there are components that inhibit the assay.
Standard Curve Range
TS4 : 1.5 to 0.024 nM ARGSVIL-peptide: 4 - 0.062 nM
12 pM

Order Details

ADAMTS4 Activity Assay Kit (Colorimetric) Summary

Aggrecanase Activity Assay Kit is a colorimetric method for assaying the activity of aggrecanase.
Standard Curve Range
TS4 : 1.5 to 0.024 nM ARGSVIL-peptide: 4 - 0.062 nM
12 pM
Assay Type
Sample Volume
100 uL
Kit Type
Activity Assay Kit (Colorimetric)


Read Publications using KA1497.

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. Wash Buffer: Bottle contains 25 ml phosphate buffer concentrate which when diluted gives0.02 M Na-phosphate buffer pH 7.2, 0.15 M NaCl, 0.05 % Tween 20.
  2. Sulfuric Acid: Bottle contains 0.25 M sulfuric acid. Ready to use
  3. Detection Solution TMB: Bottle contains 3, 3', 5, 5'-tetramethylbenzidine (TMB)/hydrogenperoxide. Ready to use
  4. ADAMTS4-Standard: Solution of 0.3 µM recombinant truncated human aggrecanase 1(ADAMTS4 amino acids F213 - A579 with C-terminal His-tag) with additives. Ready to use.
  5. Aggrecan-IGD: Tris-buffered solution of 1 µM aggrecan-IGD with additives and preservative.Ready to use
  6. Antibody-Peroxidase Conjugate: Anti-aggrecan antibody coupled to horseradish peroxidase instabilization solution. The conjugate has to be diluted 100-fold prior to use
  7. ARGSVIL-Peptide Standard: ARGSVIL-peptide at a concentration of 40 nM in EDTA-dilutionbuffer. Ready to use
  8. ELISA Buffer: Solution of 10 mg/ml bovine serum albumin, 0.15 M NaCl, 0.02 M Na-phosphatepH 7.2 with preservative. Ready to use.
  9. EDTA Dilution Buffer: Solution of 0.01 M EDTA, 10 mg/ml bovine serum albumin, 0.15 M NaCl,0.02 M Na-phosphate pH 7.2 with preservative. Ready to use
  10. Inhibitor Solution: Inhibitor concentrated with 4 mM Pefabloc, 0.01 mM pepstatin, 0.01 mMleupeptin in 10 mM MES buffer pH 6.0. Ready to use.
  11. Microtiter Plate: The plate contains 6 x 16 well strips coated with anti-neoepitope ARGSVILantibody. Ready to use.
  12. Reaction Buffer: Solution of 0.05 M Tris-HCl buffer pH 7.5, 0.15 M NaCl, 5 mM CaCl2, 10 µMZnCl2, 0.05 % Brij 35 with preservative. Ready to use


Quality control test: Standard Curve

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ADAMTS4 Activity Assay Kit (Colorimetric)

  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 4
  • ADAM metallopeptidase with thrombospondin type 1 motif, 4
  • ADAM-TS 4
  • ADAMTS-2
  • ADAM-TS4
  • ADAMTS-4
  • ADMP-1
  • ADMP-1EC
  • Aggrecanase 1
  • aggrecanase-1
  • EC 3.4.24
  • KIAA0688A disintegrin and metalloproteinase with thrombospondin motifs 4


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Gene Symbol ADAMTS4