ADAMTS4 Activity Assay Kit (Colorimetric)

ADAMTS4 Assay Kit (Colorimetric) [KA1497] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Product Discontinued

ADAMTS4 Activity Assay Kit (Colorimetric) Summary

• Description: Quality control test: Standard Curve Aggrecanase Activity Assay Kit is a colorimetric method for assaying the activity of aggrecanase.
• Standard Curve Range: TS4 : 1.5 to 0.024 nM ARGSVIL-peptide: 4 - 0.062 nM
• Sensitivity: 12 pM
• Assay Type: Colorimetric
• Sample Volume: 100 uL
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: ADAMTS4

Applications/Dilutions

• Dilutions:
  • Functional

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ADAMTS4 Activity Assay Kit (Colorimetric)

• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 4
• ADAM metallopeptidase with thrombospondin type 1 motif, 4
• ADAM-TS 4
• ADAMTS-2
• ADAMTS4
• ADAM-TS4
• ADAMTS-4
• ADMP-1
• ADMP-1EC 3.4.24.82
• Aggrecanase 1
• aggrecanase-1
• EC 3.4.24
• KIAA0688A disintegrin and metalloproteinase with thrombospondin motifs 4

Background

ADAMTS4, also known as aggrecanase-1, is a Metallopeptidase M12B type protease that is responsible for degradation of the articular cartilage protein aggrecan and the brain extracellular matrix proteoglycan brevican. The ADAMTS4 protein contains a signal sequence, a propeptide domain, a catalytic domain, a disintegrin-like motif, and a C-terminal domain with a thrombospondin (TSP) type 1 motif. ADAMTS4 has been implicated in inflammatory joint diseases, and it also may be a factor in the progression of glioma and Alzheimer's disease. ADAMTS4 expression has been documented in normal human brain, cartilage, heart, lung, placenta, muscle, and synovium. ESTs have been isolated from human tissue libraries, including normal brain, lung, pancreas, and skeletal muscle and cancerous breast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for ADAMTS4 Kit (KA1497)(3)

Publications using KA1497

• Hyemi L, Dain J, Jimin J et al. Seomae Mugwort and Jaceosidin Attenuate Osteoarthritic Cartilage Damage by Blocking I?B Degradation in Mice. J Cell Mol Med. 2020-06-11 [PMID: 32529755]
• Dai J, Zhou S, Ge Q et al. Bi-directional regulation of cartilage metabolism by inhibiting BET proteins-analysis of the effect of I-BET151 on human chondrocytes and murine joints. J Orthop Surg Res 2018-05-21 [PMID: 29784003]
• Lindner M, Thummler K, Arthur A et al. Fibroblast growth factor signalling in multiple sclerosis: inhibition of myelination and induction of pro-inflammatory environment by FGF9. Brain. 2015-04-23 [PMID: 25907862]

Bioinformatics

• Gene Symbol: ADAMTS4