ADAMTS13 Antibody - BSA Free

Western Blot: ADAMTS13 Antibody [NB100-584] - Purified recombinant Adam-TS13. Antibody used at the indicated concentrations.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ADAMTS13 Antibody - BSA Free Summary

• Description: Novus Biologicals Goat ADAMTS13 Antibody - BSA Free (NB100-584) is a polyclonal antibody validated for use in WB. Anti-ADAMTS13 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide which maps to a region between residues 250 and 350 of human A Disintegrin-like and Metalloprotease (reprolysin type) with Thrombospondin type 1 motif, 13 using the numbering given in entry XP_088414.1 (GeneID 11093).
• Specificity: NB100-584 is specific for human ADAM TS13 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADAMTS13
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:1000
• Application Notes: NB 100-584 has been shown to react with purified recombinant ADAM-TS13 in Western Blot. It has not been tested for reactivity with ADAM-TS13 from native sources, and the antibody is not warranted to have such activity. The suggested WB dilution is for recombinant protein.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ADAMTS13 Antibody - BSA Free

• A disintegrin and metalloproteinase with thrombospondin motifs 13
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 13
• ADAM metallopeptidase with thrombospondin type 1 motif, 13
• ADAMTS13
• ADAM-TS13
• ADAMTS-13
• DKFZp434C2322
• EC 3.4.24.14
• EC 3.4.24.82
• EC 3.4.24.87
• FLJ42993
• MGC118899
• MGC118900
• TTP
• TTPADAM-TS 13
• vWF-cleaving protease
• vWF-CP
• vWF-CPC9orf8
• VWFCPvon Willebrand factor-cleaving protease

Background

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura. Alternative splicing of this gene generates at least four transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAMTS13 Antibody (NB100-584)(5)

Publications using NB100-584

• Hunt RC, Geetha S, Allen CE et al. Detection of a secreted metalloprotease within the nuclei of liver cells. Mol Biosyst 2011-06-01 [PMID: 21479334]
• Chung MC, Popova TG, Jorgensen SC et al. Degradation of circulating von Willebrand factor and its regulator ADAMTS13 implicates secreted Bacillus anthracis metalloproteases in anthrax consumptive coagulopathy. J Biol Chem 2008-04-01 [PMID: 18263586]
• Tao Z, Peng Y, Nolasco L et al. Recombinant CUB-1 domain polypeptide inhibits the cleavage of ULVWF strings by ADAMTS13 under flow conditions. Blood 2005-12-01 [PMID: 16141351]
• Dong JF. Cleavage of ultra-large von Willebrand factor by ADAMTS-13 under flow conditions. J Thromb Haemost 2005-08-01 [PMID: 16102037]
• Tao Z, Wang Y, Choi H et al. Cleavage of ultralarge multimers of von Willebrand factor by C-terminal-truncated mutants of ADAMTS-13 under flow. Blood 2005-07-01 [PMID: 15774619]

Bioinformatics

• Gene Symbol: ADAMTS13
• Entrez:
  • 11093(Human)