ADAM9 Antibody [Unconjugated]

Western blot shows lysates of WI-38 human lung fibroblast cell line and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ADAM9 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF939) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for ADAM9 at approximately 110 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ADAM9 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ADAM9 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF939) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for ADAM9 at approximately 78 and 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IP, ELISA(Cap), KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ADAM9 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ADAM9
  Ala206-Asp697
  Accession # Q13443
• Specificity: Detects human ADAM9 Ectodomain in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross-reactivity with recombinant mouse (rm) rmADAM9 is observed and less than 0.05% cross-reactivity with recombinant human (rh) rhADAM8, rhADAM10, rhTACE, rhTIMP-1, rhTIMP-2, rhTIMP-3, rhTIMP-4, rhBACE-1 and rmADAM10 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADAM9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunoprecipitation 25 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human ADAM9 Ectodomain Antibody (Catalog # AF939)
  ELISA Detection: Human ADAM9 Ectodomain Biotinylated Antibody (Catalog # BAF939)
  Standard: Recombinant Human ADAM9 (Catalog # 939-AD)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM9 Antibody [Unconjugated]

• a disintegrin and metalloproteinase domain 9 (meltrin gamma)
• ADAM 9
• ADAM metallopeptidase domain 9 (meltrin gamma)
• ADAM metallopeptidase domain 9
• ADAM9
• Cellular disintegrin-related protein
• cone rod dystrophy 9
• CORD9
• disintegrin and metalloproteinase domain-containing protein 9
• EC 3.4.24
• EC 3.4.24.-
• MCMP
• MCMPMDC9KIAA0021Mltng
• MDC9
• Meltrin gamma
• Meltrin-gamma
• Metalloprotease/disintegrin/cysteine-rich protein 9
• MLTNG
• Myeloma cell metalloproteinase

Background

ADAM9, also known as MDC9 or meltrin gamma , is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1). Like other membrane-anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factor-alpha (TNF-alpha ), the p75 TNF receptor, the beta -amyloid protein precursor, and the c-kit ligand-1, implying that it may participate in shedding of these membrane proteins (2). In fact, ADAM9 has been shown to shed membrane-anchored heparin-binding EGF-like growth factor (3). In addition, it also cleaves oxidized insulin beta -chain and fibronectin (2,4). Besides its catalytic activity, ADAM9 functions as an adhesion molecule through binding of its disintegrin domain to integrins such as alpha _v beta ₅ and alpha ₆ beta ₁ (5, 6). The cytoplasmic domain of ADAM9 interacts with Src homology 3 (SH3)‑containing proteins and protein kinase C, and may mediate different signaling pathways (3, 7). ADAM9 is widely expressed in tissues (8).

1. Moss, et al. (2001) DDT 6:417.
2. Roghan, et al. (1999) J. Biol. Chem. 274:3531.
3. Izumi, et al. (1998) EMBO J. 17:7260.
4. Schwettmann and Tschesche (2001) Prot. Expre. & Purif. 21:65.
5. Nath, et al. (2000) J. Cell Sci. 113:2319.
6. Zhou, et al. (2001) Biochem. Biophys. Res. Comm. 280:574.
7. Howard, et al. (1999) J. Biol. Chem. 274:31693.
8. Weskamp, et al. (1996) J. Cell. Biol. 132:717.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM9 Antibody (AF939)(7)

Publications using AF939

• TT Le, CL Hsieh, IH Lin, CY Chu, AD Do, SH Chen, K Shigemura, K Kitagawa, M Fujisawa, MC Liu, KC Chen, SY Sung The ADAM9/UBN2/AKR1C3 axis promotes resistance to androgen-deprivation in prostate cancer American journal of cancer research, 2022-01-15;12(1):176-197. 2022-01-15 [PMID: 35141012] (Western Blot, Human)
• Jim Baggen, Hendrik Jan Thibaut, Daniel L. Hurdiss, Maryam Wahedi, Caleb D. Marceau, Arno L. W. van Vliet et al. Identification of the Cell-Surface Protease ADAM9 as an Entry Factor for Encephalomyocarditis Virus mBio 2019-08-27 [PMID: 31409686]
• M Ueno, T Shiomi, S Mochizuki, M Chijiiwa, M Shimoda, Y Kanai, F Kataoka, A Hirasawa, N Susumu, D Aoki, Y Okada ADAM9 is over-expressed in human ovarian clear cell carcinomas and suppresses cisplatin-induced cell death Cancer Sci., 2018-02-01;109(2):471-482. 2018-02-01 [PMID: 29247567] (IHC-P, Human)
• Zhenyu Cai, Anling Zhang, Swati Choksi, Weihua Li, Tao Li, Xue-Min Zhang et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration Cell Research 2016-08-01 [PMID: 27444869]
• Stokes A, Joutsa J, Ala-Aho R, Pitchers M, Pennington CJ, Martin C, Premachandra DJ, Okada Y, Peltonen J, Grenman R, James HA, Edwards DR, Kahari VM Expression profiles and clinical correlations of degradome components in the tumor microenvironment of head and neck squamous cell carcinoma. Clin. Cancer Res., 2010-03-21;16(0):2022. 2010-03-21 [PMID: 20305301] (Western Blot, Human)
• Zigrino P, Steiger J, Fox JW, Loffek S, Schild A, Nischt R, Mauch C Role of ADAM-9 disintegrin-cysteine-rich domains in human keratinocyte migration. J. Biol. Chem., 2007-08-17;282(42):30785-93. 2007-08-17 [PMID: 17704059] (ICC, IHC-Fr, Western Blot, Human)
• Franzke CW, Tasanen K, Schacke H, Tryggvason K, Mauch C, Zigrino P, Sunnarborg S, Fahrenholz F, Bruckner-Tuderman L Transmembrane collagen XVII, an epithelial adhesion protein, is shed from the cell surface by ADAMs. EMBO J., 2002-10-01;21(19):5026-35. 2002-10-01 [PMID: 12356719] (ICC, IHC-Fr, Human)

Bioinformatics

• Gene Symbol: ADAM9
• Uniprot:
  • Q13443()