ADAM22 Antibody

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ADAM22 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ADAM22
  Gly26-His528 (Leu78Val)
  Accession # Q9P0K1
• Specificity: Detects human ADAM22 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ADAM8, rhADAM12, and rhADAM33 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ADAM22
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAM22 Antibody

• ADAM 22
• ADAM metallopeptidase domain 22
• ADAM22
• MDC2
• metalloproteinase-disintegrin ADAM22-3
• metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2
• MGC149832

Background

ADAM22 (a disintegrin and metalloprotease 22; also MDC2) is an 85‑90 kDa member of the M12B peptidase family of enzymes. It is expressed on both neurons and astrocytes in cranial regions of the CNS. The human ADAM22 proprecursor is an 881 amino acid (aa) type I transmembrane protein. It contains a 197 aa cleavable proregion (aa 26‑222) and a 512 aa extracellular domain (ECD) (aa 223‑736). The ECD contains a nonfunctional metalloprotease domain (aa 239‑438), an integrin‑binding disintegrin region (aa 444‑531) and a Cys‑rich domain (aa 532‑678). Multiple splice variants exist, two of which may involve the ECD. Both show premature truncations. One has a 10 aa substitution for aa 234‑906, while a second shows an 11 aa substitution for aa 332‑906. Over aa 26‑528, human ADAM22 is 90% aa identical to mouse ADAM22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADAM22
• Uniprot:
  • Q9P0K1()