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ADAM10 Antibody (RM0146-7H12) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow
Clone
RM0146-7H12
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 647

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-12015AF350
Alexa Fluor 405
NBP2-12015AF405
Alexa Fluor 488
NBP2-12015AF488
Alexa Fluor 532
NBP2-12015AF532
Alexa Fluor 594
NBP2-12015AF594
Alexa Fluor 700
NBP2-12015AF700
Alexa Fluor 750
NBP2-12015AF750
Allophycocyanin
NBP2-12015APC
Allophycocyanin/Cy7
NBP2-12015APCCY7
Biotin
NBP2-12015B
DyLight 350
NBP2-12015UV
DyLight 405
NBP2-12015V
DyLight 488
NBP2-12015G
DyLight 550
NBP2-12015R
DyLight 650
NBP2-12015C
DyLight 680
NBP2-12015FR
DyLight 755
NBP2-12015IR
FITC
NBP2-12015F
HRP
NBP2-12015H
Janelia Fluor 549
NBP2-12015JF549
Janelia Fluor 646
NBP2-12015JF646
PE
NBP2-12015PE
PE/Atto594
NBP2-12015PEATT594
PE/Cy5.5
NBP2-12015PECY55
PE/Cy7
NBP2-12015PECY7
PerCP
NBP2-12015PCP
Unconjugated
NBP2-12015
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ADAM10 Antibody (RM0146-7H12) [Alexa Fluor® 647] Summary

Immunogen
Mouse ADAM10
Isotype
IgG2
Clonality
Monoclonal
Host
Rat
Gene
ADAM10
Purity
>95% pure by SDS-PAGE
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
>95% pure by SDS-PAGE

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ADAM10 Antibody (RM0146-7H12) [Alexa Fluor® 647]

  • a disintegrin and metalloprotease domain 10
  • a disintegrin and metalloproteinase domain 10
  • AD10
  • ADAM 10
  • ADAM metallopeptidase domain 10
  • ADAM10
  • CD156c antigen
  • CD156c
  • CDw156
  • disintegrin and metalloproteinase domain-containing protein 10
  • EC 3.4.24
  • HsT18717
  • kuz
  • Kuzbanian protein homolog
  • Kuzbanian
  • MADM
  • MADMEC 3.4.24.81
  • Mammalian disintegrin-metalloprotease

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.
Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:200 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This beta Amyloid antibody specifically stained the cells with Abeta 42/ Abeta aggregates while the normal cells were negative for abeta peptide.
Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893).
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(137612)
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Western blot shows lysates of human brain (Alzheimer's disease hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse BACE‑1 Ectodomain Monoclonal Antibody (Catalog # MAB931) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for BACE‑1 at approximately 60 and 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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TNF-alpha
[Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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Species: Hu
ADAM9 Antibody
[Unconjugated]
AF949
Western blot shows lysates of C2C12 mouse myoblast cell line and WI‑38 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ADAM9 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF949) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for ADAM9 at approximately 110 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ADAM9 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ADAM9 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF949) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for ADAM9 at approximately 78 and 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(7 Publications)
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EGFR Antibody
[Unconjugated]
AF231
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
EGFR was detected in immersion fixed frozen sections of human skin using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
Presenilin-1
Antibody (APS 18)
NB100-74510
Western Blot: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of THP-1 cell lysate.
Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of Presenilin 1 using Presenilin 1 Monoclonal antibody (APS 18) shows staining in A2058 melanoma cells. Presenilin 1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 at a dilution of 1:20-1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Prion protein
Antibody (SC57-05)
NBP2-67569
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EGF [Unconjugated]
236-EG
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1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
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ADAM15 Antibody
(23G9)
MAB935
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ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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(OTI2A6)
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Western Blot: L1CAM Antibody (2A6) [NBP2-02149] Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
Immunocytochemistry/Immunofluorescence: L1CAM Antibody (2A6) [NBP2-02149] - Staining of COS7 cells.
Immunohistochemistry-Paraffin: L1CAM Antibody (2A6) [NBP2-02149] - Staining of paraffin-embedded pancreas tissue using anti-L1CAMmouse monoclonal antibody.
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Secondary Antibodies

 

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Other Available Formats

Alexa Fluor 700 Labeled NBP2-12015AF700
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol ADAM10