ADAM10 Antibody (RM0146-7H12) - Azide and BSA Free

Immunohistochemistry-Paraffin: ADAM10 Antibody (RM0146-7H12) [NBP2-12015] - The Kidney tissue samples from the Folic Acid induced kidney injury model were fixed using 4% PFA at 4C for overnight and embeded in paraffin. 4um section was sublected to IHC with NBP2-12015

Product Discontinued

ADAM10 Antibody (RM0146-7H12) - Azide and BSA Free Summary

• Immunogen: Mouse recombinant ADAM10
• Specificity: Detects mouse ADAM10 and cross reacts with human ADAM10. Weak cross-reactivity is observed to ADAM9 and ADAM15 in Western blotting.
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: ADAM10
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:500-1000
  • Immunofluorescence 1:500-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:1000
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for ADAM10 Antibody (RM0146-7H12) - Azide and BSA Free

• a disintegrin and metalloprotease domain 10
• a disintegrin and metalloproteinase domain 10
• AD10
• ADAM 10
• ADAM metallopeptidase domain 10
• ADAM10
• CD156c antigen
• CD156c
• CDw156
• disintegrin and metalloproteinase domain-containing protein 10
• EC 3.4.24
• HsT18717
• kuz
• Kuzbanian protein homolog
• Kuzbanian
• MADM
• MADMEC 3.4.24.81
• Mammalian disintegrin-metalloprotease

Background

Proinflammatory cytokine tumor necrosis factor-alpha (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM10 Antibody (NBP2-12015)(1)

Publication using NBP2-12015

• Aung A, Cui A, Maiorino L et al. Low protease activity in B cell follicles promotes retention of intact antigens after immunization Science (New York, N.Y.) 2023-01-27 [PMID: 36701450] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: ADAM10
• Uniprot:
  • O35598()