ACY3 Antibody (OTI2D5) - Azide and BSA Free

Western Blot: ACY3 Antibody (OTI2D5) - Azide and BSA Free [NBP2-72329] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY3.

Immunohistochemistry: ACY3 Antibody (OTI2D5) - Azide and BSA Free [NBP2-72329] - Staining of paraffin-embedded Human Kidney tissue using anti-ACY3 mouse monoclonal antibody.

Flow Cytometry: ACY3 Antibody (OTI2D5) - Azide and BSA Free [NBP2-72329] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ACY3.

Flow Cytometry: ACY3 Antibody (OTI2D5) - Azide and BSA Free [NBP2-72329] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ACY3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Flow Cytometry: ACY3 Antibody (OTI2D5) - Azide and BSA Free [NBP2-72329] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ACY3 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI2D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ACY3 Antibody (OTI2D5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ACY3 Antibody (OTI2D5) - Azide and BSA Free (NBP2-03512) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ACY3 (NP_542389) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACY3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 35.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ACY3 Antibody (OTI2D5) - Azide and BSA Free

• ACY-3aspartoacylase-2
• Acylase III
• aminoacylase-3
• aspartoacylase (aminocyclase) 3
• EC 3.5.1.-
• EC 3.5.1.15
• HCBP1ASPA2
• Hepatitis C virus core-binding protein 1
• MGC9740

Background

ACY3 plays an important role in deacetylating mercapturic acids in kidney proximal tubules

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACY3