ACTR1A Antibody

Western Blot: ACTR1A Antibody [NB100-1423] - Analysis of ACTR1A in human kidney lysate using NB100-1423 at 2ug/ml (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western Blot: ACTR1A Antibody [NB100-1423] - Analysis of ACTR1A in human kidney lysate using NB100-1423 at 2ug/ml (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ACTR1A Antibody Summary

• Description: Novus Biologicals Goat ACTR1A Antibody (NB100-1423) is a polyclonal antibody validated for use in WB and ELISA. Anti-ACTR1A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-YEEDGARSIHRKT corresponding to C-Terminus according to NP_005727.1.
• Localization: Cytoplasmic
• Specificity: This epitope was selected to minimise the chance of cross-reactivity with ACTR1B (YEEDGSRAIHRKT).
• Predicted Species: Mouse (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACTR1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in human kidney lysates (calculated MW of 42.6 kDa band according to NP_005727.1).
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  ACTR1A Overexpression Lysate
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ACTR1A Antibody

• Actin-RPV
• alpha-centractin
• ARP1 (actin-related protein 1, yeast) homolog A (centractin alpha)
• ARP1 actin-related protein 1 homolog A, centractin alpha (yeast)
• ARP1actin-RPV
• centractin
• Centrosome-associated actin homolog
• CTRN1
• FLJ52695
• FLJ52800
• FLJ55002

Background

The actin-related protein ACTR1A, a member of the superfamily of actin-related proteins, is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. The major components of the dynactin complex, consisting of at least ten polypeptides, include ACTR1A. ACTR1A, the most abundant subunit in the dynactin complex, is most similar to conventional actin (>50% amino acid identity). The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1A overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of ACTR1A/dynactin complex in progression through the prometaphase of mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACTR1A Antibody (NB100-1423)(1)

Publication using NB100-1423

• Lees-Miller JP, Helfman DM, Schroer TA. A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility. Nature 1992-09-17 [PMID: 1528266]

Bioinformatics

• Gene Symbol: ACTR1A
• Entrez:
  • 10121(Human)
  • 54130(Mouse)
• Uniprot:
  • P61163(Human)