Activin C/Inhibin beta C Antibody (1077920) [Unconjugated]

Activin C was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Activin C Monoclonal Antibody (Catalog # MAB11501) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of liver hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Activin C was detected in fixed HepG2 human hepatocellular carcinoma cell line (Positive) and absent in THP‑1 human acute monocytic leukemia cell line (Negative) using Mouse Anti-Human Activin C Monoclonal Antibody (Catalog # MAB11501) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 1077920
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Activin C/Inhibin beta C Antibody (1077920) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Activin C
  Gly237-Ser352
  Accession # P55103
• Specificity: Detects human Activin C in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin C/Inhibin beta C Antibody (1077920) [Unconjugated]

• inhibin beta C chain
• Activin C
• IHBC
• INHBC
• inhibin, beta C

Background

Activins belong to the TGF-beta superfamily. They are homo- or heterodimers of Activin beta subunits. At least four mammalian beta subunits, A, B, C, and E, have been described. The beta _c subunit homodimerizes to form Activin CC. A naturally occuring Activin AC heterodimer has also been reported (1).

1. Mellor, S. et al. (2003) Endocrinology 144:4410.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P55103(Human)