Activin B/Inhibin beta B Antibody [Unconjugated]

• Reactivity: Hu
• Applications: ELISA
• Host: Goat
• Conjugate: Unconjugated

Activin B/Inhibin beta B Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human INHBB
  Accession # P09529
• Specificity: Detects human Inhibin beta B chain in direct ELISAs. In sandwich immunoassays, this antibody detects human Inhibin B when paired with the Inhibin alpha chain antibody, Catalog # MAB338.
• Source: N/A
• Isotype: IgG
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin B/Inhibin beta B Antibody [Unconjugated]

• activin AB beta polypeptide
• Activin Beta B
• Activin beta-B chain
• INHBB
• inhibin beta B chain
• Inhibin Beta B
• Inhibin Subunit Beta B
• inhibin, beta B (activin AB beta polypeptide)
• inhibin, beta B
• Inhibin, beta-2

Background

Activin and Inhibin are members of the TGF-beta superfamily of cytokines and are involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis (1‑7). Activin and Inhibin are produced as precursor proteins. Their amino terminal propeptides are proteolytically cleaved and facilitate formation of disulfide-linked dimers of the bioactive proteins (8, 9). Activins are nonglycosylated homodimers or heterodimers of various beta subunits ( beta A, beta B, beta C, and beta E in mammals), while Inhibins are heterodimers of a unique alpha subunit and one of the beta subunits. Activin A is a widely expressed homodimer of two beta A chains. The beta A subunit can also heterodimerize with a beta B or beta C subunit to form Activin AB and Activin AC, respectively (10). The 14 kDa mature human beta A chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat beta A. Activin A exerts its biological activities by binding to the type 2 serine/threonine kinase Activin RIIA which then noncovalently associates with the type 1 serine/threonine kinase Activin RIB/ALK-4 (7, 11). Signaling through this receptor complex leads to Smad activation and regulation of activin-responsive gene transcription (7, 11). The bioactivity of Activin A is regulated by a variety of mechanisms (11). BAMBI, Betaglycan, and Cripto are cell-associated molecules that function as decoy receptors or limit the ability of Activin A to induce receptor complex assembly (12‑14). The intracellular formation of Activin A can be prevented by the incorporation of the beta A subunit into Activin AC or Inhibin A (3, 10). And the bioavailability of Activin A is restricted by its incorporation into inactive complexes with alpha 2-Macroglobulin, Follistatin, and FLRG (15, 16).

1. Kumanov, P. et al. (2005) Reprod. Biomed. Online 10:786.
2. Maeshima, A. et al. (2008) Endocr. J. 55:1.
3. Rodgarkia-Dara, C. et al. (2006) Mutat. Res. 613:123.
4. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
5. Xu, P. and A.K. Hall (2006) Dev. Biol. 299:303.
6. Shav-Tal, Y. and D. Zipori (2002) Stem Cells 20:493.
7. Chen, Y.G. et al. (2006) Exp. Biol. Med. 231:534.
8. Gray, A.M. and A.J. Mason (1990) Science 247:1328.
9. Mason, A.J. et al. (1996) Mol. Endocrinol. 10:1055.
10. Thompson, T.B. et al. (2004) Mol. Cell. Endocrinol. 225:9.
11. Harrison, C.A. et al. (2005) Trends Endocrinol. Metab. 16:73.
12. Onichtchouk, D. et al. (1999) Nature 401:480.
13. Gray, P.C. et al. (2002) Mol. Cell. Endocrinol. 188:254.
14. Kelber, J.A. et al. (2008) J. Biol. Chem. 283:4490.
15. Phillips, D.J. et al. (1997) J. Endocrinol. 155:65.
16. Schneyer, A. et al. (2003) Endocrinology 144:1671.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P09529()