actin-related protein 2/3 complex subunit 1B Antibody

Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NB300-898] - Staining (2 ug/ml) of Human Liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NB300-898] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

actin-related protein 2/3 complex subunit 1B Antibody Summary

• Immunogen: Peptide with sequence AYHSFLVEPISCH corresponding to N-Terminus according to NP_005711.1.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARPC1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 2 - 4 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in human liver lysates (calculated MW of 40.9 kDa band according to NP_005711). A minor band of unknown identity was also consistently observed at 28 kDa. IHC: Paraffin embedded Human Thymus
• Control:

  actin-related protein 2/3 complex subunit 1B Overexpression Lysate

• Control Peptide:

  actin-related protein 2/3 complex subunit 1B Peptide (NB300-898PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for actin-related protein 2/3 complex subunit 1B Antibody

• actin related protein 2/3 complex, subunit 1B (41 kD)
• actin related protein 2/3 complex, subunit 1B, 41kDa
• actin-related protein 2/3 complex subunit 1B
• ARC41p41-ARCp40-ARC
• Arp2/3 complex 41 kDa subunit
• ARP2/3 protein complex subunit p41

Background

This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for actin-related protein 2/3 complex subunit 1B Antibody (NB300-898)(3)

Publications using NB300-898

• Moghe S, Jiang F, Miura Y et al. The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A. Biol Open. 2012-02-15 [PMID: 23213400]
• Molli PR, Li DQ, Bagheri-Yarmand R et al. Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A. J Cell Biol. 2010-07-12 [PMID: 20603326] (WB)
  
  Details:
  WB: Stable and transfected ZR-75 breast cancer cell lines, various figures. Note: The Arpc1b antibody was SiRNA validated by western blot, Fig 4D. The CUL3-kLHL18 ligase regulates mitotic entry and ubiquitylates, Arpc1b (IMG-3367).
• Welch MD, DePace AH, Verma S et al. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol 1997-07-28 [PMID: 9230079]

Bioinformatics

• Gene Symbol: ARPC1B
• Entrez:
  • 10095(Human)
  • 11867(Mouse)
  • 54227(Rat)
• Uniprot:
  • NP_005711.(Human)