In addition to the intermediate filaments, the cytoskeleton contains contractile micro filaments. These proteins occur in most cell types, but more abundantly in muscle cells and myoepithelial cells. There are three types of contractile proteins: actin, myosin and tubuli. This antibody reacts in immunoblots with one single protein band which is corre?sponding to actin. Antigen origin: cell preparation of chicken skeletal muscle. Antigen location: cytoplasm.
Tissue culture supernatant
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This antibody is useful in immunohistochemistry on frozen sections. Not useful for paraffin embedded tissue.Working dilution of 1:5-1:10 is advised but the optimal dilution should be tested by serial dilution. Positive control: muscle, chicken myofibrils.
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1% Sodium Azide
Tissue culture supernatant
Alternate Names for Actin Antibody (647)
actin, alpha 1, skeletal muscle
alpha skeletal muscle actin
alpha skeletal muscle
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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