Actin Antibody (647)

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Western Blot: Actin Antibody (647) [NBP1-42279] - analysis of Actin in HeLa and HEK 293 cell lysates (30ug/lane) using anti-Actin antibody. Image from verified customer review.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC, IHC-Fr
Clone
647
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Actin Antibody (647) Summary

Immunogen
The antibody reacts with the Actin antigen.
Localization
cytoplasm
Specificity
In addition to the intermediate filaments, the cytoskeleton contains contractile micro filaments. These proteins occur in most cell types, but more abundantly in muscle cells and myoepithelial cells. There are three types of contractile proteins: actin, myosin and tubuli. This antibody reacts in immunoblots with one single protein band which is corre?sponding to actin. Antigen origin: cell preparation of chicken skeletal muscle. Antigen location: cytoplasm.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
ACTA1
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
This antibody is useful in immunohistochemistry on frozen sections. Not useful for paraffin embedded tissue.Working dilution of 1:5-1:10 is advised but the optimal dilution should be tested by serial dilution. Positive control: muscle, chicken myofibrils.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-42279 in the following applications:

Reactivity Notes

Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.1% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Actin Antibody (647)

  • ACTA
  • Actin
  • actin, alpha 1, skeletal muscle
  • alpha skeletal muscle actin
  • alpha skeletal muscle
  • alpha-actin-1
  • ASMA
  • CFTD
  • CFTDM
  • MPFD
  • NEM1
  • NEM2
  • NEM3

Background

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Actin Antibody (NBP1-42279) (0)

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Review for Actin Antibody (NBP1-42279) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-42279:
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Images Ratings Applications Species Date Details
Western Blot Actin NBP1-42279
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reviewed by:
Anonymous
WB Human 10/09/2014
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Summary

ApplicationWestern Blot
Sample TestedHeLa and HEK 293 cells
SpeciesHuman

Blocking

Blocking Details4 % BSA in TBST

Primary Anitbody

Dilution RatioDiluted 1:1000 times

Secondary Antibody

Secondary DescriptionGoat Anti-Mouse IgG HRP Affinity Purified PAb (R&D Systems)
Secondary Manufacturer Cat#HAF007
Secondary ConcentrationDilution: 5,000 times

Details

Detection NotesOne-hour Western detection system (Genscript), 20 sec exposure

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Actin Antibody (NBP1-42279). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in buying an Actin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
    • I would recommend the Actin Antibody (mAbGEa) catalog # NB100-74340. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

Secondary Antibodies

 

Isotype Controls

Additional Actin Products

Actin NBP1-42279

Bioinformatics Tool for Actin Antibody (NBP1-42279)

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Blogs on Actin. Showing 1-10 of 16 blog posts - Show all blog posts.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

CRISPR/Cas9: Keep your friends close, but your viruses closer
"CRISPR", or clustered regularly interspaced short palindromic repeats, is an ancient bacterial mechanism that prevents the invasion of foreign pathogens to a host organism.  Specifically, the CRISPR sequence has been identified as a si...  Read full blog post.

Alpha-actin/ACTA1 - A skeletal muscle isoform mutated in various myopathies
Actin is an abundant cytoskeletal protein involved in a variety of cellular processes such as cell motility, cell division, and muscle contraction. Actin monomers assemble into filaments and can provide a track for transport of cargo by the molecul...  Read full blog post.

Alpha-Adducin - Assembling the cytoskeleton meshwork that underlies the plasma membrane
The structure and organization of the plasma membrane is maintained by an underlying network of cytoskeletal proteins including actin and spectrin. Adducin, a member of this protein network, binds to bundles and caps actin filaments and links them...  Read full blog post.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Actin Dynamics and Endocytic Trafficking
Actin is a ubiquitous and an essential component of the cytoskeleton, with critical roles in a wide range of cellular processes. It is abundant protein whose monomers polymerize into polarized actin filaments, within epithelial cells. Filamentous acti...  Read full blog post.

Beta Actin is a Key Player in Embryonic Development and Cell Motility
Actins are an essential component of the cytoskeleton, with critical roles in a wide range of cellular processes, including cell migration, division, and regulation of gene expression. These functions are attributed to the ability of actin to form fil...  Read full blog post.

"Actin the Fool" about Cytoskeleton Structure
Actins are highly conserved, commonly found and abundant proteins involved in several types of cell motility as well as cytoskeleton maintenance. In vertebrate species, three main groups of actin isoforms, the alpha, beta and gamma, have been identifi...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

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Recent Reviews

5
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1
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Anonymous
10/09/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACTA1