Actin Alpha 1 Cardiac Muscle Antibody (22D3) [mFluor Violet 500 SE]

• Reactivity: Hu, Mu, Rt, Po, Bv, Gt, Rb, Ze
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 22D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 500 SE

Actin Alpha 1 Cardiac Muscle Antibody (22D3) [mFluor Violet 500 SE] Summary

• Immunogen: Derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of Actin Alpha 1 Cardiac Muscle with an acetylated N-terminus (Ac-DDEETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
• Specificity: This antibody is highly specific for actin alpha 1 (cardiac), and does not cross react with other actin isoforms.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTC1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

The epitope recognized by this antibody is highly conserved; the antibody therefore cross-reacts with many species

Bovine reactivity reported in scientific literature (PMID: 30591979). Mouse reactivity reported in scientific literature (PMID: 28624189).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Actin Alpha 1 Cardiac Muscle Antibody (22D3) [mFluor Violet 500 SE]

• ACTC
• ACTC1
• actin, alpha, cardiac muscle 1
• alpha-Cardiac Actin
• ASD5
• Cardiac alpha-Actin
• CMD1R
• CMH11
• LVNC4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTC1