Mouse, Rat ACTH ELISA Kit (Colorimetric) Summary
| Description |
ACTH (Mouse/Rat) ELISA Kit (Chemiluminescence) is an ultra sensitive method (Less than 1 pg/mL) used for the quantitative determination of ACTH (Adrenocorticotropic Hormone) in mouse/rat plasma. |
| Standard Curve Range |
7 to 515 pg/mL |
| Sensitivity |
1 pg/mL |
| Assay Type |
Competitive ELISA |
| Sample Volume |
200 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
POMC |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
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- ACTH Standard Zero: Ready to use
- ACTH Standard: Lyophilized
- Biotinylated ACTH Antibody (Reagent 1)
- Enzyme labeled ACTH Antibody (Reagent 2)
- Luminol buffer
- Luminol substrate, 3X
- Microwells coated with Streptavidin
- Sample Diluent
- Wash Concentrate (Reagent A)
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse, Rat ACTH ELISA Kit (Colorimetric)
Background
ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for ACTH ELISA Kit (KA0917)(9)
Showing Publications 1 -
9 of 9.
| Publications using KA0917 |
Applications |
Species |
| Yang H, Xia L, Chen J et al. Stress-glucocorticoid-TSC22D3 axis compromises therapy-induced antitumor immunity. Nat Med. 2019-09-09 [PMID: 31501614] |
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| Spychala A, Ruther U FTO affects hippocampal function by regulation of BDNF processing. PLoS One. 2019-02-07 [PMID: 30730976] |
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| Tadaishi M, Toriba Y, Shimizu M et al. Adenosine stimulates hepatic glycogenolysis via adrenal glands-liver crosstalk in mice. PLoS One. 2018-12-21 [PMID: 30576384] |
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| Sominsky L, Ziko I, Spencer SJ. et al. Neonatal overfeeding disrupts pituitary ghrelin signalling in female rats long-term; Implications for the stress response. PLoS One 2017-03-10 [PMID: 28282447] |
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| Ye L, Hu Z, Wang H et al. Tris-(2,3-Dibromopropyl) Isocyanurate, a New Emerging Pollutant, Impairs Cognition and Provokes Depression-Like Behaviors in Adult Rats. PLoS One 2015-10-12 [PMID: 26458255] |
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| Khalil MS. Vitamin D3 May Ameliorate the Ketoconazole Induced Adrenal Injury: Histological and Immunohistochemical Studies on Albino Rats. Acta Histochem Cytochem 2015-08-27 [PMID: 26379312] |
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| Schneeberger M, Gomez-Valades AG, Altirriba J et al. Reduced alpha-MSH Underlies Hypothalamic ER-Stress-Induced Hepatic Gluconeogenesis. Cell Reports. 2015-07-01 [PMID: 26166568] |
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| Maity-Kumar G, Thal DR, Baumann B et al. Neuronal redox imbalance results in altered energy homeostasis and early postnatal lethality. FASEB J 2015-03-31 [PMID: 25829510] |
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| Swierczynska MM, Mateska I, Peitzsch M et al. Changes in morphology and function of adrenal cortex in mice fed a high-fat diet. Int J Obes (Lond) 2014-06-12 [PMID: 24919565] |
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Product General Protocols
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FAQs for ACTH ELISA Kit (KA0917). (Showing 1 - 1 of 1 FAQs).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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