ACTH Antibody (CLIP/1407) - Azide and BSA Free

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, IHC-P, CyTOF-ready, ICC/IF
Clone
CLIP/1407
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

ACTH Antibody (CLIP/1407) - Azide and BSA Free Summary

Immunogen
Synthetic peptide corresponding to aa25-39 of human ACTH (NGAEDESAEAFPLEF)
Localization
Cytoplasmic
Marker
Pituitary Marker
Specificity
ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to CLIP (aa25-39 of ACTH); does not react with Synacthen (aa1-24 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
POMC
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 0.5-1ug/million cells
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • CyTOF-ready
  • Immunofluorescence 1-2ug/ml
Application Notes
Hu Chromosome Location: 2p23.3
Mol. Weight of Antigen: ACTH is ~5kDa, and the POMC precursor is ~30kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for ACTH Antibody (CLIP/1407) - Azide and BSA Free

  • ACTH
  • adrenocorticotropic hormone
  • Adrenocorticotropin
  • alpha-melanocyte-stimulating hormone
  • alpha-MSH
  • beta-endorphin
  • beta-LPH
  • beta-melanocyte-stimulating hormone
  • beta-MSH
  • CLIP
  • Corticotropin
  • corticotropin-like intermediary peptide
  • corticotropin-lipotropin
  • gamma-LPH
  • gamma-MSH
  • lipotropin beta
  • lipotropin gamma
  • LPH
  • melanotropin alpha
  • melanotropin beta
  • melanotropin gamma
  • met-enkephalin
  • MSH
  • NPP
  • POC
  • pro-ACTH-endorphin
  • proopiomelanocortin preproprotein
  • proopiomelanocortin
  • pro-opiomelanocortin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol POMC