ACTH Antibody (AH26)

Immunohistochemistry-Paraffin: ACTH Antibody (AH26) [NBP2-29452] - Formalin-paraffin human Pituitary Gland stained with ACTH ab (AH26).

• Reactivity: Hu, Mu, Rt
• Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: AH26
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ACTH Antibody (AH26) Summary

• Immunogen: Synthetic peptide corresponding to aa1-24 of human ACTH
• Localization: Cytoplasm
• Marker: Pituitary Marker
• Specificity: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).AIt also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POMC
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
• Application Notes: The molecular weight of ACTH is 5 kDa, and the POMC precursor is ~30 kDa. The molecular weight of POMC may vary depending on isoform variation and post-translational modifications.
  Use in Immunohistochemistry-Frozen has been reported in literature. Use in ELISA reported in scientific literature (PMID: 30181699).
  Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Reactivity Notes

Expected to show broad species reactivity

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for ACTH Antibody (AH26)

• ACTH
• adrenocorticotropic hormone
• Adrenocorticotropin
• alpha-melanocyte-stimulating hormone
• alpha-MSH
• beta-endorphin
• beta-LPH
• beta-melanocyte-stimulating hormone
• beta-MSH
• CLIP
• Corticotropin
• corticotropin-like intermediary peptide
• corticotropin-lipotropin
• gamma-LPH
• gamma-MSH
• lipotropin beta
• lipotropin gamma
• LPH
• melanotropin alpha
• melanotropin beta
• melanotropin gamma
• met-enkephalin
• MSH
• NPP
• POC
• pro-ACTH-endorphin
• proopiomelanocortin preproprotein
• proopiomelanocortin
• pro-opiomelanocortin

Background

ACTH (Adrenocorticotrophic hormone) is produced and secreted by the anterior pituitary gland, and is a key component of the hypothalamic-pituitary-adrenal axis. ACTH is synthesized from the precursor molecule pre-opiomelanocorin (POMC). POMC undergoes proteolytic cleavages and processing to generate not only ACTH but also a number of other small biologically active peptides including alpha-MSH and beta-endorphin, all in cell type specific manners. The production of ACTH is triggered by biological stress. ACTH, in turn, then stimulates the secretion of corticosteroids by the adrenal cortex. The half-life of ACTH in human blood is only about ten minutes. An excess of ACTH can cause Cushing's syndrome whereas ACTH deficiency can result in secondary adrenal insufficiency. ACTH consists of 39 amino acids (aa), and can be proteolytically cleaved into different active fragments including CLIP (corticotropin-like intermediate lobe peptide), consisting of aa 17-39, which stimulates insulin production. Synacthen is an active synthetic form of ACTH, corresponding to the first 24 aa, which is used to conduct ACTH stimulation tests.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACTH Antibody (NBP2-29452)(1)

Publication using NBP2-29452

• Lee B, Shim I, Lee H et al. Tetramethylpyrazine reverses anxiety-like behaviors in a rat model of post-traumatic stress disorder Korean J Physiol Pharmacol Aug 29 2018 [PMID: 30181699] (ELISA, Rat)

FAQs for ACTH Antibody (NBP2-29452). (Showing 1 - 2 of 2 FAQ).

1. Has the cross-reactivity of NBP2-29452 with POMC been assessed?
   • This antibody was validated for specificity and epitope mapped by the inventor of the clone. This antibody is specific for Synacthen (aa1-24 of ACTH) and does not react with CLIP (aa17-39 of ACTH). The antibody is able to detect both ACTH and POMC.
2. How the reactivity of NBP2-29452 with ACTH been assessed?
   • We unfortunately do not have access to this initial testing data from the inventor.

Bioinformatics

• Gene Symbol: POMC
• Uniprot:
  • P01189()