ACSS1 Antibody - Azide and BSA Free

Western Blot: ACSS1 Antibody [NBP2-92202] - Analysis of extracts of various cell lines, using ACSS1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

Immunocytochemistry/Immunofluorescence: ACSS1 Antibody [NBP2-92202] - Analysis of L929 cells using ACSS1 at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: ACSS1 Antibody [NBP2-92202] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using ACSS1 Rabbit pAb (NBP2-92202) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ACSS1 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 340-689 of human ACSS1 (NP_115890.2). ITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACSS1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for ACSS1 Antibody - Azide and BSA Free

• ACAS2L
• AceCS2
• AceCS2L
• Acetate--CoA ligase 2
• Acetyl-CoA synthetase 2
• acetyl-Coenzyme A synthetase 2 (AMP forming)-like
• acetyl-coenzyme A synthetase 2-like, mitochondrial
• acyl-CoA synthetase short-chain family member 1dJ568C11.3
• EC 6.2.1
• EC 6.2.1.1
• FLJ45659
• KIAA1846
• MGC33843

Background

An acetyl-CoA synthetase found in the mitochondrial matrix has been characterized in both cow and mouse. It is primarily a cardiac enzyme which produces acetyl-CoA mainly for the oxidation of acetate. The protein encoded by this gene is structurally similar to the cow and mouse proteins and therefore may serve a similar function in humans. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: ACSS1