Acetylcholinesterase/ACHE Antibody

Product Discontinued

Acetylcholinesterase/ACHE Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 560-600 of Human AChE.
• Marker: Early Neuronal Development Marker
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACHE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Immunocytochemistry/Immunofluorescence was reported in scientific literature.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Acetylcholinesterase/ACHE Antibody

• acetylcholinesterase (Yt blood group)
• Acetylcholinesterase
• ACHE
• apoptosis-related acetylcholinesterase
• ARACHE
• EC 3.1.1
• EC 3.1.1.7
• N-ACHE
• Yt blood group
• YT

Background

Acetylcholinesterase (AChE) hydrolyzes acetylcholine at synaptic junctions.Alternative mRNA splicing gives rise to three forms of AChE. The T form, also known as the asymmetric form, is soluble and is present in synapses. The H form is also known as the globular form and is present on the outer surfaces of cell membranes. The R form is not known to be a functional species. AChE globular form subunits are GPI-anchored to cell membranes and asymmetric subunits are anchored to basal lamina components by a collagen tail. The catalytic subunits of AChE are oligomers composed of disulfide-linked homodimers.The loss of AChE from cholinergic and noncholinergic neurons in the brain is seen in patients with Alzheimer's disease. However, AChE activity is increased around amyloid plaques, which may be due to a disturbance in calcium homeostasis involving the opening of L-type voltage-dependent calcium channels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Acetylcholinesterase/ACHE Antibody (NBP1-51274)(3)

Publications using NBP1-51274

• Kim JW, Seung H, Kwon KJ et al. Subchronic treatment of donepezil rescues impaired social, hyperactive, and stereotypic behavior in valproic Acid-induced animal model of autism PLoS ONE 2014-08-19 [PMID: 25133713]
• El-Akabawy G, El-Kholy W. Neuroprotective effect of ginger in the brain of streptozotocin-induced diabetic rats. Ann. Anat. 2014-03-12 [PMID: 24680376] (IHC-P, Rat)
• Nemazanyy I, Blaauw B, Paolini C et al. Defects of Vps15 in skeletal muscles lead to autophagic vacuolar myopathy and lysosomal disease. EMBO Mol Med 2013-04-30 [PMID: 23630012] (IHC-P, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: ACHE
• Entrez:
  • 43(Human)
  • 11423(Mouse)
  • 83817(Rat)
• Uniprot:
  • P22303(Human)