Acetylcholinesterase/ACHE Antibody

Images

 
Western Blot: Acetylcholinesterase/ACHE Antibody [NB600-911] - Analysis using the Biotin conjugate of NB600-911. Detection of antibody with pyranose oxidase under reducing (R) and non-reducing (NR) conditions. Reduced ...read more

Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Acetylcholinesterase/ACHE Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Acetyl Cholinesterase [Bovine Erythrocytes]
Localization
Cell Membrane and Secreted
Marker
Early Neuronal Development Marker
Specificity
Acetylcholine Esterase
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACHE
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of Acetyl Cholinesterase [Bovine Erythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:1,200 - 1:5,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Cross-reacts with Cow. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Acetylcholinesterase/ACHE Antibody

  • acetylcholinesterase (Yt blood group)
  • Acetylcholinesterase
  • ACHE
  • apoptosis-related acetylcholinesterase
  • ARACHE
  • EC 3.1.1
  • EC 3.1.1.7
  • N-ACHE
  • Yt blood group
  • YT

Background

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF9024
Species: Mu, Rt
Applications: WB, IHC
NBP1-30052
Species: Hu, Mu, Rt, Av, Ch, GP, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP2-13306
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-32108
Species: Hu, Rt
Applications: WB, ICC/IF
NBP2-45897
Species: Hu
Applications: WB, IHC
NBP2-13075
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
MAB4375
Species: Hu, Mu, Rt
Applications: IHC
NBP1-89726
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-49118
Species: Hu
Applications: IHC, IHC-P
NBP1-85740
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
H00010908-M08
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-05163
Species: Hu, Rt, Po, Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for Acetylcholinesterase/ACHE Antibody (NB600-911) (0)

There are no publications for Acetylcholinesterase/ACHE Antibody (NB600-911).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Acetylcholinesterase/ACHE Antibody (NB600-911) (0)

There are no reviews for Acetylcholinesterase/ACHE Antibody (NB600-911). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Acetylcholinesterase/ACHE Antibody (NB600-911) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Acetylcholinesterase/ACHE Products

Bioinformatics Tool for Acetylcholinesterase/ACHE Antibody (NB600-911)

Discover related pathways, diseases and genes to Acetylcholinesterase/ACHE Antibody (NB600-911). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Acetylcholinesterase/ACHE Antibody (NB600-911)

Discover more about diseases related to Acetylcholinesterase/ACHE Antibody (NB600-911).
 

Pathways for Acetylcholinesterase/ACHE Antibody (NB600-911)

View related products by pathway.

PTMs for Acetylcholinesterase/ACHE Antibody (NB600-911)

Learn more about PTMs related to Acetylcholinesterase/ACHE Antibody (NB600-911).
 

Research Areas for Acetylcholinesterase/ACHE Antibody (NB600-911)

Find related products by research area.

Blogs on Acetylcholinesterase/ACHE

There are no specific blogs for Acetylcholinesterase/ACHE, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Acetylcholinesterase/ACHE Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ACHE
Entrez