ACBD3 Antibody (OTI1G2) - Azide and BSA Free

Western Blot: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACBD3.

Immunohistochemistry: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of Human pancreas tissue.

Flow Cytometry: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by ACBD3 antibody, and then analyzed by flow cytometry.

Western Blot: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of Adenocarcinoma of Human breast tissue.

Immunohistochemistry: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of Adenocarcinoma of Human endometrium tissue.

Immunohistochemistry: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunohistochemistry: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of Carcinoma of Human lung tissue.

Immunohistochemistry: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of Human breast tissue.

Immunohistochemistry: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of Human colon tissue.

Immunohistochemistry: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of Human endometrium tissue.

Immunohistochemistry: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of Human Kidney tissue.

Immunohistochemistry: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of Human liver tissue.

Immunohistochemistry: ACBD3 Antibody (OTI1G2) - Azide and BSA Free [NBP2-72148] - Analysis of Human prostate tissue.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI1G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ACBD3 Antibody (OTI1G2) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human ACBD3(NP_073572) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACBD3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 60.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ACBD3 Antibody (OTI1G2) - Azide and BSA Free

• acyl-CoA binding domain containing 3
• Acyl-CoA-binding domain-containing protein 3
• acyl-Coenzyme A binding domain containing 3
• GCP60Golgi phosphoprotein 1
• GOCAP1Peripheral benzodiazepine receptor-associated protein PAP7
• golgi complex associated protein 1, 60kDa
• Golgi complex-associated protein 1
• Golgi resident protein GCP60
• GOLPH1PBR- and PKA-associated protein 7
• PAP7
• PBR associated protein
• peripherial benzodiazepine receptor associated protein
• PKA (RIalpha)-associated protein

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in the maintenance of Golgi structure and function through its interaction with the integral membrane protein giantin. It may also be involved in the hormonal regulation of steroid formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACBD3