ACADM Antibody (1E5G10)

Western Blot: ACADM Antibody (1E5G10) [NBP3-16437] - Western blot analysis of extracts of various cell lines, using ACADM Rabbit mAb (NBP3-16437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry/Immunofluorescence: ACADM Antibody (1E5G10) [NBP3-16437] - Immunofluorescence analysis of NIH-3T3 cells using ACADM Rabbit mAb (NBP3-16437) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: ACADM Antibody (1E5G10) [NBP3-16437] - Immunohistochemistry of paraffin-embedded mouse kidney using ACADM Rabbit mAb (NBP3-16437) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: ACADM Antibody (1E5G10) [NBP3-16437] - Immunohistochemistry of paraffin-embedded human colon using ACADM Rabbit mAb (NBP3-16437) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1E5G10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ACADM Antibody (1E5G10) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ACADM (P11310). WYFLLARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEAT
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACADM
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:400
  • Immunohistochemistry 1:200 - 1:800
  • Immunohistochemistry-Paraffin 1:200 - 1:800
  • Western Blot 1:1000 - 1:6000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ACADM Antibody (1E5G10)

• ACAD1
• acyl-CoA dehydrogenase, C-4 to C-12 straight chain
• acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• EC 1.3.99
• EC 1.3.99.3
• FLJ18227
• FLJ99884
• MCADFLJ93013
• MCADH
• medium-chain specific acyl-CoA dehydrogenase, mitochondrial

Background

ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increased. Clinical phenotypes are associated with ACADM hereditary deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACADM