Abraxas Antibody

Images

 
Immunohistochemistry: Abraxas Antibody [NBP1-22978] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Abraxas used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB staining ...read more
Immunohistochemistry: Abraxas Antibody [NBP1-22978] - Sample: FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
Immunoprecipitation: Abraxas Antibody [NBP1-22978] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-Abraxas antibody used for IP at 10 mcg/mg lysate. Antibody was also immunoprecipitated by rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, IP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Abraxas Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 400 and 409 of human Abraxas (coiled-coil domain containing 98) using the numbering given in entry NP_620775.2 (GeneID 84142).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FAM175A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 5-15 ug/mg lysate
Application Notes
It is not recommended for western analysis.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Abraxas Antibody

  • BRCA1-A complex subunit Abraxas
  • ABRA1
  • CCDC98
  • family with sequence similarity 175, member A

Background

Abraxas was identified in a phosphopeptide affinity proteomic and mass spec analysis as a protein that bound directly to the BRCA1 BRCT repeat. Along with RAP80, Abraxas was required to form a complex with BRCA1 for the DNA damage response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FAM175A
Entrez
Uniprot