ABO, Blood Group B Antigen Antibody (HEB-29)

Agglutination: ABO, Blood Group B Antigen Antibody (HEB-29) [NB120-2524] - Agglutination of particular blood groups using mouse monoclonal HEB-29 (anti-blood group B).

• Reactivity: Hu
• Applications: Func, IHC, Func
• Clone: HEB-29
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

ABO, Blood Group B Antigen Antibody (HEB-29) Summary

• Immunogen: Mixture of erythrocytes of blood group B and glycoprotein fraction isolated from the saliva of secretors with blood group B.
• Specificity: The antibody HEB-29 reacts with human blood group B. The specifity of the antibody HEB-29 was confirmed by comparison of specifity and reactivity to standard reagent using >5.000 samples of blood.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABO
• Purity: Concentrated Supernatant

Applications/Dilutions

• Dilutions:
  • Agglutination
  • Aggregation
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: In agglutination tests, the minimum titre is 1:128 with erythrocytes of blood group B.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: The hybridoma culture supernatant is 4 x concentrated by ultrafiltration using 100 kDa-cut off membrane.
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Concentrated Supernatant

Alternate Names for ABO, Blood Group B Antigen Antibody (HEB-29)

• A transferase
• A3GALNT
• A3GALT1
• ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase;transferase B, alpha 1-3-galactosyltransferase)
• ABO glycosyltransferase
• B transferase
• B(A) alpha-1,3-galactosyltransferase
• EC 2.4.1.37
• EC 2.4.1.40
• Fucosylglycoprotein 3-alpha-galactosyltransferase
• Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
• Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
• Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
• GTB
• Histo-blood group A transferase
• histo-blood group A2 transferase
• histo-blood group ABO system transferase
• Histo-blood group B transferase
• NAGAT

Background

Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABO, Blood Group B Antigen Antibody (NB120-2524)(2)

Publications using NB120-2524

• Vanak J, Drimalova D, Smyslova O, Nemec M, Viklicky V, Wisniewski K. : Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma;36(4):479-88. 1989-01-01 [PMID: 2475797]
• Nemec M, Drimalova D, Horejsi V, Vanak J, Bartek J, Viklicky V. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang;52(1-2):125-8. 1987-01-01 [PMID: 2440181]

Bioinformatics

• Gene Symbol: ABO
• Entrez:
  • 28(Human)
• Uniprot:
  • P16442(Human)