ABO, Blood Group A Antigen Antibody (HE-14) - Azide and BSA Free

Product Discontinued

ABO, Blood Group A Antigen Antibody (HE-14) - Azide and BSA Free Summary

• Description: Purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS WITHOUT BSA & azide at 1.0 mg/ml.
• Immunogen: Mixture of erythrocytes of blood group A1and glyco protein fraction isolated from the saliva of secretors with blood group A
• Localization: Cell Surface
• Specificity: This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 Ab was used.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABO
• Purity: Protein L purified

Applications/Dilutions

• Dilutions:
  • Agglutination
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: ELISA: For coating, order Ab without BSA.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: No Preservative
• Concentration: 0.2 mg/ml
• Purity: Protein L purified

Alternate Names for ABO, Blood Group A Antigen Antibody (HE-14) - Azide and BSA Free

• A transferase
• A3GALNT
• A3GALT1
• ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase;transferase B, alpha 1-3-galactosyltransferase)
• ABO glycosyltransferase
• B transferase
• B(A) alpha-1,3-galactosyltransferase
• EC 2.4.1.37
• EC 2.4.1.40
• Fucosylglycoprotein 3-alpha-galactosyltransferase
• Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
• Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
• Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
• GTB
• Histo-blood group A transferase
• histo-blood group A2 transferase
• histo-blood group ABO system transferase
• Histo-blood group B transferase
• NAGAT

Background

Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABO
• Uniprot:
  • P16442(Human)