ABL2 Overexpression Lysate (Native)

Images

 
Western Blot: ABL2 Overexpression Lysate (Adult Normal) [NBL1-07210] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ABL2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ABL2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC213232 and based on accession number NM_007314. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene) (ABL2), transcript variant b, mRNA.
Gene
ABL2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
128.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ABL2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ABL2 Overexpression Lysate (Native)

  • Abelson murine leukemia viral (v-abl) oncogene homolog 2
  • Abelson murine leukemia viral oncogene homolog 2
  • Abelson-related gene protein
  • Abelson-relatedgene)
  • arg tyrosine kinase
  • EC 2.7.10
  • EC 2.7.10.2
  • FLJ22224
  • FLJ31718
  • FLJ41441
  • tyrosine-protein kinase ABL2
  • Tyrosine-protein kinase ARG
  • v-abl Abelson murine leukemia viral oncogene homolog 2

Background

This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinase. The protein is highly similar to the ABL1 protein, including the tyrosine kinase, SH2 and SH3 domains, and has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ETV6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (b) encodes the longest isoform (b, also known as 1BLCTL). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ABL2 Lysate (NBL1-07210) (0)

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Video Protocols

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Bioinformatics

Gene Symbol ABL2