ABI1 Antibody (1013203) [Unconjugated]

ABI1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human ABI1 Monoclonal Antibody (Catalog # MAB10249) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ABI1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) tissue using Mouse Anti-Human ABI1 Monoclonal Antibody (Catalog # MAB10249) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 1013203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ABI1 Antibody (1013203) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human ABI1
  Val429-Asp508
  Accession # Q8IZP0
• Specificity: Detects human ABI1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ABI1 Antibody (1013203) [Unconjugated]

• Abelson interactor 1
• ABI1
• ABI-1
• abl interactor 1
• Abl-binding protein 4
• ABLBP4
• abl-interactor 1
• Abl-interactor protein 1 long
• E3B1
• eps8 binding protein
• Eps8 SH3 domain-binding protein
• Eps8-binding protein
• interactor protein AblBP4
• nap1 binding protein
• Nap1-binding protein
• NAP1BP
• spectrin SH3 domain binding protein 1
• Spectrin SH3 domain-binding protein 1
• SSH3BP
• SSH3BP1

Background

Abelson Interactor 1 (ABI1) is an adaptor protein involved in cytoskeletal reorganization and EGFR signaling. Overexpression of ABI1 inhibits EGF-induced activation of Erks which negatively regulate cell growth and transformation. In the brain, ABI1 seems to regulate dendritic outgrowth and determines the shape and number of synaptic contacts of developing neurons.

1. UniProt: The Universal Protein Knowledgebase. (2017)  Nucleic Acids Res. 45: D158-D169.
2. Fan. P.-D. and S.P. Goff (2000) Mol. Cell. Biol. 20:7591.
3. Xiong X. et al. (2008) Biochim Biophy. Acta 1783:737.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8IZP0()