ABCF1 Antibody (SN07-12)

Western Blot: ABCF1 Antibody (SN07-12) [NBP2-67698] - Analysis of ABCF1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate Predicted band size: 96 kDa Observed band size: 105 kDa

Immunocytochemistry/Immunofluorescence: ABCF1 Antibody (SN07-12) [NBP2-67698] - ICC staining ABCF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ABCF1 Antibody (SN07-12) [NBP2-67698] - Analysis of paraffin-embedded human stomach tissue using anti-ABCF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: ABCF1 Antibody (SN07-12) [NBP2-67698] - Analysis of ABCF1 on Hela lysates using anti-ABCF1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: ABCF1 Antibody (SN07-12) [NBP2-67698] - Staining ABCF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ABCF1 Antibody (SN07-12) [NBP2-67698] - Staining ABCF1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ABCF1 Antibody (SN07-12) [NBP2-67698] - Analysis of paraffin-embedded human colon cancer tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ABCF1 Antibody (SN07-12) [NBP2-67698] - Analysis of paraffin-embedded human kidney tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ABCF1 Antibody (SN07-12) [NBP2-67698] - Analysis of paraffin-embedded human liver cancer tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ABCF1 Antibody (SN07-12) [NBP2-67698] - Analysis of paraffin-embedded rat stomach tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ABCF1 Antibody (SN07-12) [NBP2-67698] - Analysis of paraffin-embedded rat kidney tissue using anti-ABCF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: ABCF1 Antibody (SN07-12) [NBP2-67698] - Analysis of paraffin-embedded human prostate tissue using anti-ABCF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

ABCF1 Antibody (SN07-12) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ABCF1 aa 796-845 / 845. (SwissProt: Q8NE71 Human; SwissProt: Q6MG08 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ABCF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ABCF1 Antibody (SN07-12)

• ABC27
• ABC50TNFalpha-inducible ATP-binding protein
• ATP-binding cassette 50 (TNF-alpha stimulated)
• ATP-binding cassette 50
• ATP-binding cassette sub-family F member 1
• ATP-binding cassette, sub-family F (GCN20), member 1
• EC 2.7.7.6
• EC 3.6.3.17
• EST123147
• TNF-alpha-stimulated ABC protein

Background

ABCF1 is encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABCF1