Lysozyme Antibody (EPR2994(2))

Product Discontinued

Lysozyme Antibody (EPR2994(2)) Summary

• Immunogen: A synthetic peptide corresponding to residues in human Lysozyme C was used as an immunogen.
• Marker: Histiocytic Marker
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LYZ
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500-1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-5000
  • Immunoprecipitation 1:10-100
  • Western Blot 1:10000-50000

Reactivity Notes

Mouse cross reactivity tested by western blot and Immunohistochemistry. Rat cross reactivity tested by Western Blot only.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Lysozyme Antibody (EPR2994(2))

• EC 3.2.1.17
• lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C
• lysozyme C
• Lysozyme
• LZM
• renal amyloidosis

Background

Lysozyme is a ubiquitous enzyme defined as muraminidase catalyzing the hydrolysis of the beta (1-4) glycosidic bond in bacterial peptidoglycan, a major component of the bacterial cell wall (1). Lysozyme C in tissues and body fluids is associated with the monocyte-macrophage system and enhances the activity of immunoagents. Lysozyme C catalyzes the hydrolysis of certain mucopolysaccharides of bacterial cell walls. Specifically, it catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. It is found in the spleen, lung, kidney, white blood cells, plasma, saliva, milk, and tears (2). Defects in Lysozyme C are a cause of amyloidosis type 8 (AMYL8), also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lysozyme Antibody (NBP1-95509)(5)

Publications using NBP1-95509

• Cotton JL, Dang K, Hu L et al. PTEN and LKB1 are differentially required in Gli1-expressing mesenchymal cells to suppress gastrointestinal polyposis Cell reports 2022-07-19 [PMID: 35858546] (IF/IHC, Mouse)
  
  Details:
  Figure S5
• Li Q, Sun Y, Jarugumilli GK et al. Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription Cell Stem Cell 2020-04-02 [PMID: 32259481]
• Cotton JL, Li Q, Ma L et al. YAP/TAZ and Hedgehog Coordinate Growth and Patterning in Gastrointestinal Mesenchyme Dev. Cell 2017-09-19 [PMID: 28943241] (Human)
• Tashakori M, Zhang Y, Xiong S et al. p53 Activity Dominates That of p73 upon Mdm4 Loss in Development and Tumorigenesis. Mol. Cancer Res. 2016-01-01 [PMID: 26527653] (IF/IHC, Mouse)
• LOrinc E, Mellblom L, Oberg S. The immunophenotypic relationship between the submucosal gland unit, columnar metaplasia and squamous islands in the columnar lined oesophagus Histopathology 2015-04-21 [PMID: 25898799] (IF/IHC, Human)

FAQs for Lysozyme Antibody (NBP1-95509). (Showing 1 - 1 of 1 FAQs).

1. Could you let me know the range of immunogen used to generate the NBP1-95509?
   • The immunogen sequence for NBP1-95509 lies within amino acids 45-78 of the human lysozyme protein.

Bioinformatics

• Gene Symbol: LYZ
• Entrez:
  • 4069(Human)
• Uniprot:
  • P61626(Human)